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Literature summary for 1.2.1.50 extracted from

  • Willis, R.M.; Wahlen, B.D.; Seefeldt, L.C.; Barney, B.M.
    Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol (2011), Biochemistry, 50, 10550-10558.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
plasmid pPCRMALD8 containing the FACoAR from Marinobacter aquaeolei VT8 with an N-terminal maltose binding protein fusion and a C-terminal His-tag transformed into the Escherichia coli TB1 strain for protein expression Marinobacter nauticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
SDS-PAGE, in agreement with mass calculated from amino acid sequence Marinobacter nauticus

Organism

Organism UniProt Comment Textmining
Marinobacter nauticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by amylose affinity chromatography and metal affinity chromatography with nickel Marinobacter nauticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonoyl-CoA + NADPH + H+
-
Marinobacter nauticus (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenal + CoA + NADP+ further reduction of (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenal into the corresponding (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraen-1-ol ?
fatty acyl-CoA + NADPH + H+
-
Marinobacter nauticus fatty aldehyde + CoA + NADP+ reaction intermediate, enzyme converts fatty acyl-CoA directly into the corresponding fatty alcohol by four-electron reduction, free fatty aldehyde intermediate is not accessible ?
lauroyl-CoA + NADPH + H+
-
Marinobacter nauticus dodecanal + CoA + NADP+ further reduction of dodecanal into the corresponding dodecan-1-ol ?
additional information no reaction product detected with NADH or free fatty acids Marinobacter nauticus ?
-
?
myristoyl-CoA + NADPH + H+
-
Marinobacter nauticus tetradecanal + CoA + NADP+ further reduction of tetradecanal into the corresponding tetradecan-1-ol ?
octanoyl-CoA + NADPH + H+
-
Marinobacter nauticus octanal + NADP+ further reduction of octanal into the corresponding octan-1-ol ?
oleoyl-CoA + NADPH + H+
-
Marinobacter nauticus (9Z)-octadec-9-enal + CoA + NADP+ further reduction of (9Z)-octadec-9-enal into the corresponding (9Z)-octadec-9-en-1-ol ?
palmitoleoyl-CoA + NADPH + H+
-
Marinobacter nauticus (9Z)-hexadec-9-enal + CoA + NADP+ further reduction of (9Z)-hexadec-9-enal into the corresponding (9Z)-hexadec-9-en-1-ol ?
palmitoyl-CoA + NADPH + H+
-
Marinobacter nauticus hexadecanal + CoA + NADP+ further reduction of hexadecanal into the corresponding hexadecan-1-ol ?
stearoyl-CoA + NADPH + H+
-
Marinobacter nauticus octadecanal + CoA + NADP+ further reduction of octadecanal into the corresponding octadecan-1-ol ?

Synonyms

Synonyms Comment Organism
FACoAR
-
Marinobacter nauticus
fatty acyl-CoA reductase
-
Marinobacter nauticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Marinobacter nauticus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 activity falling dramatically below pH 6.5 and above 9.0 Marinobacter nauticus