Cloned (Comment) | Organism |
---|---|
plasmid pPCRMALD8 containing the FACoAR from Marinobacter aquaeolei VT8 with an N-terminal maltose binding protein fusion and a C-terminal His-tag transformed into the Escherichia coli TB1 strain for protein expression | Marinobacter nauticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
116000 | - |
SDS-PAGE, in agreement with mass calculated from amino acid sequence | Marinobacter nauticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marinobacter nauticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by amylose affinity chromatography and metal affinity chromatography with nickel | Marinobacter nauticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenal + CoA + NADP+ | further reduction of (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenal into the corresponding (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraen-1-ol | ? | |
fatty acyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | fatty aldehyde + CoA + NADP+ | reaction intermediate, enzyme converts fatty acyl-CoA directly into the corresponding fatty alcohol by four-electron reduction, free fatty aldehyde intermediate is not accessible | ? | |
lauroyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | dodecanal + CoA + NADP+ | further reduction of dodecanal into the corresponding dodecan-1-ol | ? | |
additional information | no reaction product detected with NADH or free fatty acids | Marinobacter nauticus | ? | - |
? | |
myristoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | tetradecanal + CoA + NADP+ | further reduction of tetradecanal into the corresponding tetradecan-1-ol | ? | |
octanoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | octanal + NADP+ | further reduction of octanal into the corresponding octan-1-ol | ? | |
oleoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | (9Z)-octadec-9-enal + CoA + NADP+ | further reduction of (9Z)-octadec-9-enal into the corresponding (9Z)-octadec-9-en-1-ol | ? | |
palmitoleoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | (9Z)-hexadec-9-enal + CoA + NADP+ | further reduction of (9Z)-hexadec-9-enal into the corresponding (9Z)-hexadec-9-en-1-ol | ? | |
palmitoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | hexadecanal + CoA + NADP+ | further reduction of hexadecanal into the corresponding hexadecan-1-ol | ? | |
stearoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | octadecanal + CoA + NADP+ | further reduction of octadecanal into the corresponding octadecan-1-ol | ? |
Synonyms | Comment | Organism |
---|---|---|
FACoAR | - |
Marinobacter nauticus |
fatty acyl-CoA reductase | - |
Marinobacter nauticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Marinobacter nauticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | activity falling dramatically below pH 6.5 and above 9.0 | Marinobacter nauticus |