Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Disulfiram | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00417 | - |
4-trimethylaminobutyraldehyde | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bos taurus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
3 * 55000, SDS-PAGE | Bos taurus |
160000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-trimethylaminobutyraldehyde + NAD+ | Bos taurus | function in carnitine biosynthesis | 4-trimethylaminobutyrate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using column chromatography on blue dextran-Sepharose and 5'-AMP-Sepharose | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
activity on propionaldehyde | Bos taurus |
5.1 | - |
activity on 4-trimethylaminobutyraldehyde | Bos taurus |
Storage Stability | Organism |
---|---|
-80°C, 0.1% 2-mercaptoethanol, 20% glycerol, several months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-trimethylaminobutyraldehyde + NAD+ | - |
Bos taurus | 4-trimethylaminobutyrate + NADH + H+ | - |
? | |
4-trimethylaminobutyraldehyde + NAD+ | function in carnitine biosynthesis | Bos taurus | 4-trimethylaminobutyrate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | low activity | Bos taurus | acetate + NADH + H+ | - |
? | |
butyraldehyde + NAD+ | low activity | Bos taurus | butyrate + NADH | - |
? | |
propionaldehyde + NAD+ | low activity | Bos taurus | propionate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 55000, SDS-PAGE | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
0.1% 2-mercaptoethanol, 20% glycerol, fairly stable, but storage for several weeks results in loss of most of the activity | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | 9.8 | - |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 10.5 | pH 6.2: about 30% of activity maximum, pH 10.5: about 15% of activity maximum | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | absolute requirement for | Bos taurus |