Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme from Pseudomonas putida is an exception in that it does not depend on glutathione for catalysis | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
gene fdhA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)/pG-TF2 | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 15 mg/ml FDH protein and 4 mg/ml NAD+ in 10 mM Tris-HCl, pH 8.0, and 1 mM DTT, with 0.001 ml of reservoir solution containing 0.1 M Bis-Tris, pH 5.5, and 1.8 M ammonium sulfate, at 18°C, method optimization, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement method, and modelling | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | Pseudomonas aeruginosa | - |
formate + NADH + 2 H+ | - |
? | |
formaldehyde + NAD+ + H2O | Pseudomonas aeruginosa LESB58 | - |
formate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HTE3 | - |
- |
Pseudomonas aeruginosa LESB58 | Q9HTE3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)/pG-TF2 by nickel affinity chromatography dialysis, anion exchange chromatography, and dialysis against crystallization buffer | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
formaldehyde + NAD+ + H2O = formate + NADH + H+ | Pseudomonas aeruginosa FDH has a conserved glutathione-independent ping pong mechanism of formaldehyde oxidization | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | - |
Pseudomonas aeruginosa | formate + NADH + 2 H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Pseudomonas aeruginosa LESB58 | formate + NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure modelling and analysis | Pseudomonas aeruginosa |
tetramer | the active form with each monomer (350-400 amino-acid residues) consisting of catalytic and coenzyme binding domains together with two bound zinc ions | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Pseudomonas aeruginosa |
fdhA | gene name, UniProt | Pseudomonas aeruginosa |
glutathione-independent formaldehyde dehydrogenase | - |
Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+-binding mode analysis, with two bound zinc ions per monomer of the tetrameric enzyme | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
evolution | formaldehyde dehydrogenase (FDH) is a member of the zinc-containing medium-chain alcohol dehydrogenase family | Pseudomonas aeruginosa |
physiological function | the enzyme oxidizes toxic formaldehyde to formate using NAD+ as an electron carrier | Pseudomonas aeruginosa |