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Literature summary for 1.2.1.44 extracted from

  • Li, J.; Fan, F.; Wang, L.; Zhan, Q.; Wu, P.; Du, J.; Yang, X.; Liu, Y.
    Cloning and expression analysis of cinnamoyl-CoA reductase (CCR) genes in sorghum (2016), PeerJ, 4, e2005 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.44

Cloned(Commentary)

Cloned (Comment) Organism
gene SbCCR1, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH5alpha, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthaminana leaves via Agrobacterum tumefaciens transfection, quantitative real-time PCR enzyme expression analysis Sorghum bicolor
gene SbCCR2-1, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH5alpha, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthaminana leaves via Agrobacterum tumefaciens transfection, quantitative real-time PCR enzyme expression analysis Sorghum bicolor
gene SbCCR2-2, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH5alpha, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthaminana leaves via Agrobacterum tumefaciens transfection, quantitative real-time PCR enzyme expression analysis Sorghum bicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Sorghum bicolor 5737
-

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
-
-
Sorghum bicolor C5XWV7
-
-
Sorghum bicolor C5YLL4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Sorghum bicolor
-
additional information the highest expression levels of the three CCR genes occur in stem. The expression levels of SbCCR1 and SbCCR2-2 in stem and root are higher than in spikelet and leaf, but those of SbCCR2-1 in stem and spikelet are higher than in leaf and root. The gene copy number of SbCCR1 is significantly higher than those of SbCCR2-1 and SbCCR2-2 in root, leaf, stem and spikelet, especially in stem Sorghum bicolor
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root
-
 Sorghum bicolor
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spikelet
-
 Sorghum bicolor
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stem
-
 Sorghum bicolor
-

Subunits

Subunits Comment Organism
? x * 37950, about, sequence calculation Sorghum bicolor
? x * 38210, about, sequence calculation Sorghum bicolor
? x * 40240, about, sequence calculation Sorghum bicolor
More the enzyme contains an NADP binding domain, an NWYCY domain, and a substrate domain Sorghum bicolor

Synonyms

Synonyms Comment Organism
CCR
-
 Sorghum bicolor
SbCCR1
-
 Sorghum bicolor
SbCCR2-1
-
 Sorghum bicolor
SbCCR2-2
-
 Sorghum bicolor

pI Value

Organism Comment pI Value Maximum pI Value
Sorghum bicolor sequence calculation
-
 5.47
Sorghum bicolor sequence calculation
-
 6.5
Sorghum bicolor sequence calculation
-
 8.79

Expression

Organism Comment Expression
Sorghum bicolor the enzyme is induced by drought treatment up
Sorghum bicolor the enzyme is induced by drought treatment, genes SbCCR1 and SbCCR2-2 mainly respond in the first phase of drought defense, while the SbCCR2-1 gene responds during the whole drought defense period up

General Information

General Information Comment Organism
metabolism cinnamoyl-CoA reductase (CCR) is the first enzyme in the monolignol-specific branch of the lignin biosynthetic pathway Sorghum bicolor
physiological function the enzyme is involved in drought defense Sorghum bicolor