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Literature summary for 1.2.1.44 extracted from

  • Escamilla-Trevino, L.L.; Shen, H.; Uppalapati, S.R.; Ray, T.; Tang, Y.; Hernandez, T.; Yin, Y.; Xu, Y.; Dixon, R.A.
    Switchgrass (Panicum virgatum) possesses a divergent family of cinnamoyl CoA reductases with distinct biochemical properties (2009), New Phytol., 185, 143-155.
    View publication on PubMed
Search for more literature for 1.2.1.44

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression as His-tagged fusion protein in Escherichia coli Panicum virgatum

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxyferuloyl-CoA substrate inhibition at high concentration above 60 microM; substrate inhibition at high concentration above 60 microM Panicum virgatum
feruloyl-CoA substrate inhibition at high concentration above 60 microM; substrate inhibition at high concentration above 60 microM Panicum virgatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0254
-
 feruloyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.0296
-
 caffeoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.0316
-
 4-coumaroyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.0376
-
 caffeoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.047
-
 feruloyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.0548
-
 sinapoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.0565
-
 sinapoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.0572
-
 4-coumaroyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37880
-
 predicted Panicum virgatum
39040
-
 predicted Panicum virgatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaroyl-CoA + NADPH + H+ Panicum virgatum
-
 4-coumaroylaldehyde + CoA + NADP+
-
 r
4-coumaroyl-CoA + NADPH + H+ Panicum virgatum preferenced substrate 4-coumaroylaldehyde + CoA + NADP+
-
 r
5-hydroxyferuloyl-CoA + NADPH + H+ Panicum virgatum
-
 5-hydroxyferulic aldehyde + CoA + NADP+
-
 r
caffeoyl-CoA + NADPH + H+ Panicum virgatum
-
 caffeolylaldehyde + CoA + NADP+
-
 r
caffeoyl-CoA + NADPH + H+ Panicum virgatum preferenced substrate caffeolylaldehyde + CoA + NADP+
-
 r
feruloyl-CoA + NADPH + H+ Panicum virgatum
-
 coniferaldehyde + CoA + NADP+
-
 r
feruloyl-CoA + NADPH + H+ Panicum virgatum preferred substrate coniferaldehyde + CoA + NADP+
-
 r
additional information Panicum virgatum based on its properties and expression pattern, PvCCR1 is probably associated with lignin biosynthesis during plant development and is therefore a target for the engineering of improved biomass, whereas PvCCR2 may function in defense ?
-
 ?
additional information Panicum virgatum PvCCR1 is probably associated with lignin biosynthesis during plant development ?
-
 ?
sinapoyl-CoA + NADPH + H+ Panicum virgatum very low efficiency sinapaldehyde + CoA + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Panicum virgatum D2IX40
-
-
Panicum virgatum D2IX45
-
-

Purification (Commentary)

Purification (Comment) Organism
as His-tagged enzyme by means of Ni-NTA beads Panicum virgatum

Source Tissue

Source Tissue Comment Organism Textmining
internode
-
 Panicum virgatum
-
leaf
-
 Panicum virgatum
-
node
-
 Panicum virgatum
-
seedling Alamo cultivar Panicum virgatum
-
stem
-
 Panicum virgatum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + NADPH + H+
-
 Panicum virgatum 4-coumaroylaldehyde + CoA + NADP+
-
 r
4-coumaroyl-CoA + NADPH + H+ preferenced substrate Panicum virgatum 4-coumaroylaldehyde + CoA + NADP+
-
 r
5-hydroxyferuloyl-CoA + NADPH + H+
-
 Panicum virgatum 5-hydroxyferulic aldehyde + CoA + NADP+
-
 r
caffeoyl-CoA + NADPH + H+
-
 Panicum virgatum caffeolylaldehyde + CoA + NADP+
-
 r
caffeoyl-CoA + NADPH + H+ preferenced substrate Panicum virgatum caffeolylaldehyde + CoA + NADP+
-
 r
feruloyl-CoA + NADPH + H+
-
 Panicum virgatum coniferaldehyde + CoA + NADP+
-
 r
feruloyl-CoA + NADPH + H+ preferred substrate Panicum virgatum coniferaldehyde + CoA + NADP+
-
 r
additional information based on its properties and expression pattern, PvCCR1 is probably associated with lignin biosynthesis during plant development and is therefore a target for the engineering of improved biomass, whereas PvCCR2 may function in defense Panicum virgatum ?
-
 ?
additional information PvCCR1 is probably associated with lignin biosynthesis during plant development Panicum virgatum ?
-
 ?
sinapoyl-CoA + NADPH + H+ very low efficiency Panicum virgatum sinapaldehyde + CoA + NADP+
-
 r

Synonyms

Synonyms Comment Organism
CCR ambiguous Panicum virgatum
cinnamoyl CoA reductase
-
 Panicum virgatum
PvCCR1
-
 Panicum virgatum
PvCCR2
-
 Panicum virgatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Panicum virgatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 sinapoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.063
-
 feruloyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.162
-
 caffeoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.188
-
 4-coumaroyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.2
-
 sinapoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.23
-
 caffeoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
0.32
-
 4-coumaroyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
2.01
-
 feruloyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
 assay at Panicum virgatum

pI Value

Organism Comment pI Value Maximum pI Value
Panicum virgatum predicted
-
 6
Panicum virgatum predicted
-
 6.6