Literature summary for 1.2.1.44 extracted from

Li, L.; Cheng, X.; Lu, S.; Nakatsubo, T.; Umezawa, T.; Chiang, V.L.
Clarification of cinnamoyl co-enzyme A reductase catalysis in monolignol biosynthesis of Aspen (2005), Plant Cell Physiol., 46, 1073-1082.

Search for more literature for 1.2.1.44

Cloned(Commentary)

Cloned (Comment)	Organism
into the pET41a+ and pET23b+ vector for expression in Escherichia coli BL21DE3 cells	Populus tremuloides

Inhibitors

Inhibitors	Comment	Organism	Structure
5-hydroxyferuloyl-CoA	competitive inhibitor	Populus tremuloides
caffeoyl-CoA	competitive inhibitor	Populus tremuloides
feruloyl-CoA	competitive inhibitor	Populus tremuloides
sinapoyl-CoA	competitive inhibitor	Populus tremuloides

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0137	-	feruloyl-CoA	-	Populus tremuloides
0.0763	-	caffeoyl-CoA	-	Populus tremuloides
0.099	-	5-hydroxyferuloyl-CoA	-	Populus tremuloides
0.1045	-	sinapoyl-CoA	-	Populus tremuloides

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cinnamaldehyde + CoA + NADP+	Populus tremuloides	-	cinnamoyl-CoA + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Populus tremuloides	Q9M631	-	-

Purification (Commentary)

Purification (Comment)	Organism
CCR protein is purified using a Bug-Buster GSTclosed circleBind purification kit, and the fused tag is cleaved and removed using the Enterokinase Cleavage capture kit	Populus tremuloides

Source Tissue

Source Tissue	Comment	Organism	Textmining
xylem	-	Populus tremuloides	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.01	-	feruloyl-CoA as a substrate at pH 7.0	Populus tremuloides
0.02	-	feruloyl-CoA as a substrate at pH 6.5	Populus tremuloides
0.1	-	feruloyl-CoA as a substrate at pH 5.0	Populus tremuloides
0.12	-	feruloyl-CoA as a substrate at pH 5.5	Populus tremuloides
0.14	-	feruloyl-CoA as a substrate at pH 6.0	Populus tremuloides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-hydroxyferuloyl-CoA + NADPH + H+	-	Populus tremuloides	5-hydroxyconiferaldehyde + CoA + NADP+	-	?
caffeoyl-CoA + NADPH + H+	-	Populus tremuloides	caffealdehyde + CoA + NADP+	-	?
cinnamaldehyde + CoA + NADP+	-	Populus tremuloides	cinnamoyl-CoA + NADPH + H+	-	?
feruloyl-CoA + NADPH + H+	-	Populus tremuloides	coniferaldehyde + CoA + NADP+	-	?
p-coumaroyl-CoA + NADPH + H+	-	Populus tremuloides	p-coumaraldehyde + CoA + NADP+	-	?
sinapoyl-CoA + NADPH + H+	-	Populus tremuloides	sinapaldehyde + CoA + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
CCR	ambiguous	Populus tremuloides
cinnamoyl-Co-enzyme A reductase	-	Populus tremuloides
cinnamoyl-CoA reductase	-	Populus tremuloides
PtCCR	-	Populus tremuloides

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.7	-	caffeoyl-CoA	-	Populus tremuloides
4.9	-	5-hydroxyferuloyl-CoA	-	Populus tremuloides
5.3	-	sinapoyl-CoA	-	Populus tremuloides
7.8	-	feruloyl-CoA	-	Populus tremuloides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	activity assay, feruloyl-CoA as substrate	Populus tremuloides

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	6.5	when the pH is above 6.5, the activity becomes negligible, feruloyl-CoA as substrate	Populus tremuloides

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Populus tremuloides

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0063	-	feruloyl-CoA	competitive inhibition of feruloyl-CoA on the 5-hydroxyferuloyl-CoA reaction	Populus tremuloides
0.0066	-	feruloyl-CoA	competitive inhibition of feruloyl-CoA on the sinapoyl-CoA reaction	Populus tremuloides
0.0083	-	feruloyl-CoA	competitive inhibition of feruloyl-CoA on the caffeoyl-CoA reaction	Populus tremuloides
0.0153	-	caffeoyl-CoA	competitive inhibition of caffeoyl-CoA on the feruloyl-CoA reaction	Populus tremuloides
0.0157	-	5-hydroxyferuloyl-CoA	competitive inhibition of 5-hydroxyferuloyl-CoA on the feruloyl-CoA reaction	Populus tremuloides
0.02	-	sinapoyl-CoA	competitive inhibition of sinapoyl-CoA on the feruloyl-CoA reaction	Populus tremuloides

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Release 2023.1 (January 2023)