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Literature summary for 1.2.1.44 extracted from

  • Wengenmayer, H.; Ebel, J.; Grisebach, H.
    Enzymic synthesis of lignin precursors. Purification and properties of a cinnamoyl-CoA: NADPH reductase from cell suspension cultures of soybean (Glycine max) (1976), Eur. J. Biochem., 65, 529-536.
    View publication on PubMed
Search for more literature for 1.2.1.44

General Stability

General Stability Organism
2-mercaptoethanol: stabilizes , only stable in presence of Glycine max
ethyleneglycol, 15%, stabilizes Glycine max

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.01 mM, 50% inhibition Glycine max
CoASH product inhibition Glycine max
iodoacetamide 1 mM, 50% inhibition Glycine max
NADP+ product inhibition Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 NADPH cosubstrate feruloyl-CoA Glycine max
0.05
-
 5-hydroxyferuloyl-CoA
-
 Glycine max
0.073
-
 feruloyl-CoA
-
 Glycine max
0.1
-
 p-Coumaroyl-CoA
-
 Glycine max
0.12
-
 NADPH cosubstrate sinapoyl-CoA Glycine max
0.2
-
 caffeoyl-CoA
-
 Glycine max
0.29
-
 NADPH cosubstrate p-coumaroyl-CoA Glycine max
0.4
-
 sinapoyl-CoA
-
 Glycine max
0.9
-
 cinnamoyl-CoA
-
 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 36000 SDS-PAGE Glycine max
38000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
 L. var. Mandarin, soybean
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.2
-
-
 Glycine max

Storage Stability

Storage Stability Organism
-20°C, 100 mM Tris-HCl buffer, pH 7.5, 10% ethyleneglycol, several weeks Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxyferuloyl-CoA + NADPH + H+ i.e. 4,5-dihydroxy-3-methoxycinnamoyl-CoA Glycine max 5-hydroxyferulic aldehyde + CoA + NADP+
-
 ?
caffeoyl-CoA + NADPH
-
 Glycine max caffeic aldehyde + CoA + NADP+
-
 ?
cinnamoyl-CoA + NADPH
-
 Glycine max cinnamaldehyde + CoA + NADP+
-
 ?
feruloyl-CoA + NADPH
-
 Glycine max ferulic aldehyde + CoA + NADP+
-
 ?
p-coumaroyl-CoA + NADPH
-
 Glycine max p-coumaric aldehyde + CoA + NADP+
-
 ?
sinapoyl-CoA + NADPH
-
 Glycine max sinapic aldehyde + CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000 SDS-PAGE Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.2
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
5.4 8 pH 5.4: about 10% of activity maximum, pH 8.0: about 15% of activity maximum Glycine max

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 30°C, 30 min, most stable around Glycine max

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Glycine max