Cloned (Comment) | Organism |
---|---|
gene FAR2, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3), transient expression of GFP-tagged FAR2 in Nicotiana benthamiana leaves under control of CaMV 35S promoter via Agrobacterium tumefaciens strain V3101::pMP90 transfection method | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00531 | - |
hexadecanal | pH 7.0, 30°C, recombinant enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | enzyme AtFAR2 is targeted to chloroplasts | Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexadecanal + CoA + NAD+ | Arabidopsis thaliana | - |
hexadecanoyl-CoA + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q08891 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexadecanal + CoA + NAD+ | - |
Arabidopsis thaliana | hexadecanoyl-CoA + NADH + H+ | - |
? | |
additional information | Arabidopsis thaliana AtFAR2 has the ability to use both, C16:0-ACP and C16:0-CoA substrates for the production of C16:0- alcohol, purified enzyme AtFAR2 is specific for acyl-ACP substrates, AtFAR2 shows the highest activity towards C16:0-CoA, approximately 11times higher in comparison to the activity towards C18:0-CoA. AtFAR2 displays a very low activity with C18:1-CoA, and application of C14:0-CoA as a substrate generates only aldehyde and free fatty acid | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtFAR2 | - |
Arabidopsis thaliana |
FAR2 | - |
Arabidopsis thaliana |
MS2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
low activity with alcohols and free fatty acids occurs at above pH 9.0 | Arabidopsis thaliana |
6 | - |
with aldehyde substrates | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | AtFAR2 is highly similar in properties and substrate specificity to AtFAR6 | Arabidopsis thaliana |
malfunction | mutations in specific genes encoding fatty acid reductases (FAR) responsible for fatty alcohol production cause abnormal development of pollen. A disrupted AtFAR2 (MS2) gene in Arabidopsis thaliana results in pollen developing an abnormal exine layer and a reduced fertility phenotype | Arabidopsis thaliana |
physiological function | AtFAR2 is a fatty acid reductase from Arabidopsis thaliana that reduces fatty acyl-CoA and -ACP substrates into fatty alcohols, cf. EC 1.2.1.80. Enzyme AtFAR2 is the major enzyme responsible for exine layer functionality, AtFAR6 might provide functional redundancy to AtFAR2 | Arabidopsis thaliana |