Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(Phosphonoacetylamido)-L-alanine | competitive with respect to DL-pyrroline-5-carboxylic acid, non-competitive with respect to NADP+ and phosphate | Escherichia coli | |
NADPH | competitive with respect to all three substrates | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km with various phosphate substitutes | Escherichia coli | |
0.05 | - |
NADP+ | - |
Escherichia coli | |
0.35 | - |
phosphate | - |
Escherichia coli | |
2.5 | - |
L-glutamate 5-semialdehyde | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-5-glutamyl phosphate + NADPH | Escherichia coli | second enzyme in pathway of proline biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-5-glutamyl phosphate + NADPH | second enzyme in pathway of proline biosynthesis | Escherichia coli | ? | - |
? | |
L-glutamate 5-semialdehyde + phosphate + NADP+ | divalent anions can substitute for phosphate | Escherichia coli | L-5-glutamyl phosphate + NADPH | - |
r | |
L-glutamate 5-semialdehyde + phosphate + NADP+ | L-glutamate 5-semialdehyde is in equilibrium with delta'-pyrroline-5-carboxylate | Escherichia coli | L-5-glutamyl phosphate + NADPH | - |
r | |
L-glutamate 5-semialdehyde + phosphate + NADP+ | - |
Escherichia coli | gamma-glutamyl phosphate + NADPH + H+ | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | less active than NADPH | Escherichia coli | |
NADPH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
NADPH | - |
Escherichia coli | |
22.5 | - |
3-(Phosphonoacetylamido)-L-alanine | - |
Escherichia coli |