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Literature summary for 1.2.1.38 extracted from

  • Cherney, L.T.; Cherney, M.M.; Garen, C.R.; Niu, C.; Moradian, F.; James, M.N.
    Crystal structure of N-acetyl-gamma-glutamyl-phosphate reductase from Mycobacterium tuberculosis in complex with NADP(+) (2007), J. Mol. Biol., 367, 1357-1369.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
AGPR apoenzyme and AGPR in complex with NADP+ crystallized by the sitting-drop method. Crystals of the apoenzyme belong to space group P212121 with four protein subunits in the asymmetric unit (a crystallographic tetramer) and to space group C2 having two protein subunits in the asymmetric unit (a crystallographic C2-dimer). Each AGPR subunit consists of alpha/beta and alpha + beta domains. NADP+ is bound in the cleft between them. Conformational change in AGPR upon NADP+ binding, a loop (Leu88 to His92) moves more than 5 A to confine sterically the cofactor's adenine moiety in a hydrophobic pocket. Residues His217 and His219 can form hydrogen bonds with the docked substrate. An ion pair can form between the substrate phosphate group and the guanidinium group of Arg114, which optimally places and orients the substrate for subsequent nucleophilic attack by Cys158 on the substrate gamma-carboxyl group Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPZ9
-
-
Mycobacterium tuberculosis H37Rv P9WPZ9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-5-glutamyl phosphate + NADPH + H+
-
Mycobacterium tuberculosis N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
-
?
N-acetyl-5-glutamyl phosphate + NADPH + H+
-
Mycobacterium tuberculosis H37Rv N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
-
?

Subunits

Subunits Comment Organism
dimer crystallography Mycobacterium tuberculosis
tetramer crystallography Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
AGPR
-
Mycobacterium tuberculosis