Application | Comment | Organism |
---|---|---|
additional information | rapid, highly sensitive, and reproducible coupled enzyme assays for AGS, AGK, and GAT using recombinant Escherichia coli AGK and AGPR as coupling enzymes | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
ligated into pET15b vector. Overexpressed as N-terminal His6 tag in Escherichia coli BL21 (DE3) cells carrying pLysS | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P11446 | - |
- |
Purification (Comment) | Organism |
---|---|
by His tag affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-5-glutamyl phosphate + NADPH + H+ | substrate produced by AGK | Escherichia coli | N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AGPR | - |
Escherichia coli |
N-acetylglutamate 5-phosphate reductase | - |
Escherichia coli |