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Literature summary for 1.2.1.36 extracted from

  • Belyaeva, O.V.; Korkina, O.V.; Stetsenko, A.V.; Kedishvili, N.Y.
    Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase/reductase with a retinaldehyde reductase activity (2008), FEBS J., 275, 138-147.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Homo sapiens
glutathione
-
Homo sapiens

Application

Application Comment Organism
additional information RDH13 is significantly different from related RDH11, RDH12 and RDH14 in that it is targeted to the mitochondria, but at the same time, RDH13 is very similar to the members of the RDH11-14 cluster of short-chain dehydrogenases/reductases in terms of its substrate and cofactor preferences. It may function to protect mitochondria against oxidative stress associated with the highly reactive retinaldehyde produced from dietary beta-carotene Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the pET28a vector. Expressed in Sf9 cells as a fusion with the C-terminal His6 tag Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information if dithiothreitol is omitted from the elution buffer during RDH13-His6 purification, the purified enzyme has a very low activity, but can be reactivated by the addition of dithiothreitol. Becomes partially inactivated after 20 min of incubation at 37°C. Not inhibited at 0.05 mM by nonanal, 6-cis-nonenal and 2-trans-nonenal, glyceraldehyde and acetoacetyl-coenzyme A, taurocholic acid, 25-hydroxycholesterol and 25-nor-5-cholesten-3-ol-25b-one Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
NADPH
-
Homo sapiens
0.003
-
all-trans-retinol
-
Homo sapiens
0.0032
-
all-trans-retinaldehyde
-
Homo sapiens
6
-
NADH
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane outer side Homo sapiens 5743
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
immunoblotting Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
RDH13-His6 purified by Ni2+ affinity chromatography, to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
heart abundant Homo sapiens
-
kidney abundant Homo sapiens
-
LNCaP cell expresses endogenous RDH13 at high levels Homo sapiens
-
lung abundant Homo sapiens
-
ovary
-
Homo sapiens
-
prostate
-
Homo sapiens
-
testis
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.047
-
-
Homo sapiens
0.13
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-80°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinaldehyde + NAD(P)H
-
Homo sapiens NAD(P)+ + H2O + all-trans-retinoate
-
?
all-trans-retinol + NAD(P)H
-
Homo sapiens NAD(P)+ + H2O + all-trans-retinaldehyde
-
?
additional information no significant conversion of 17beta-, 3alpha- and 11beta-hydroxysteroids, and corresponding ketosteroids Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
RDH13
-
Homo sapiens
retinol dehydrogenase 13
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH
-
Homo sapiens
NADPH prefers NADPH to NADH as a cofactor Homo sapiens