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Literature summary for 1.2.1.36 extracted from
- Purification and partial characterization of a rat kidney aldehyde dehydrogenase that oxidizes retinal to retinoic acid (1993), Biochem. Cell Biol., 71, 85-89.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| retinal | strong substrate inhibition with 0.02 mM or more | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0085 | - |
retinal | retinal bovine serum albumin, Km is 25 times lower than the Km for acetaldehyde | Rattus norvegicus | |
| 0.0104 | - |
retinal | retinal-Tween-80 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
3 * 53000, SDS-PAGE | Rattus norvegicus |
| 140000 | - |
standard deviation 3500, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| retinal + NAD+ + H2O | Rattus norvegicus | - |
retinoate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| retinal + NAD+ + H2O = retinoate + NADH + H+ | participates in vitamin A metabolism | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.267 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, at concentration of 0.078 mg/ml, 15% glycerol, stable for three months | Rattus norvegicus |
| dilution (1:5) with 100 mM phosphate buffer often results in loss of 80-90% of specific activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NAD+ + H2O | no substrate inhibition | Rattus norvegicus | acetate + NADH + H+ | - |
? | |
| retinal + NAD+ + H2O | - |
Rattus norvegicus | retinoate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 53000, SDS-PAGE | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | 8.6 | on chromatofocusing column, major activity peak is eluted at pH range 8.4-8.6 | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | NAD+ dependent | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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