Cloned (Comment) | Organism |
---|---|
expression of isozyme type-2 in Escherichia coli strain BL21(DE3) as N-terminally fusion protein to glutathione-S-transferase | Mus musculus |
recombinant in Escherichia coli BL21(DE3) | Mus musculus |
General Stability | Organism |
---|---|
DTT stabilizes isozyme type-2 | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citral | complete inhibition at 1 mM; strongly suppress enzyme reaction | Mus musculus | |
additional information | all-trans-retinol and chloral hydrate do not inhibit enzyme reaction | Mus musculus | |
p-hydroxymercuribenzoate | complete inhibition at 1 mM in absence of DTT, 2 mM DTT protect nearly completely; strongly suppress enzyme reaction, reversed by addition of DTT | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis, isozyme type-2 | Mus musculus | |
0.0006 | - |
13-cis-retinal | - |
Mus musculus | |
0.00062 | - |
13-cis-retinal | recombinant isozyme type-2, pH 9.0, 25°C | Mus musculus | |
0.00066 | - |
all-trans-retinal | recombinant isozyme type-2, pH 9.0, 25°C | Mus musculus | |
0.0007 | - |
all-trans-retinal | - |
Mus musculus | |
0.00225 | - |
9-cis-retinal | recombinant isozyme type-2, pH 9.0, 25°C | Mus musculus | |
0.0023 | - |
9-cis-retinal | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulate the type-2 isozyme by 40% at 2 mM MgCl2 | Mus musculus | |
MgCl2 | 40% stimulation of enzyme activity with 2 mM MgCl2 | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Mus musculus |
55000 | - |
x * 55000, recombinant detagged isozyme type-2, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
13-cis-retinal + NAD+ + H2O | Mus musculus | type-2 isozyme | 13-cis-retinoate + NADH | - |
? | |
9-cis-retinal + NAD+ + H2O | Mus musculus | type-2 isozyme | 9-cis-retinoate + NADH | - |
? | |
all-trans-retinal + NAD+ + H2O | Mus musculus | type-2 isozyme | all-trans-retinoate + NADH | - |
? | |
additional information | Mus musculus | type-2 isozyme catalyzes the last step in biosynthesis of retinoic acid in embryos and adult reproductive organs, isozyme type-2 regulates the retinoic acid level during embryogenesis and tissue differentiation | ? | - |
? | |
retinal + NAD+ + H2O | Mus musculus | - |
retinoate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus | - |
type-2 isozyme | - |
Purification (Comment) | Organism |
---|---|
after recombinant production in Escherichia coli BL-21DE3 | Mus musculus |
recombinant isozyme type-2 as fusion protein from Escherichia coli by GST-affinity chromatography, cleavage by thrombin to eliminate the fusion tag | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
retinal + NAD+ + H2O = retinoate + NADH + H+ | sulfhydryl group involved in activity | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | midgestational | Mus musculus | - |
additional information | specific expression in embryos and adult reproductive organs | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
13-cis-retinal + NAD+ + H2O | type-2 isozyme | Mus musculus | 13-cis-retinoate + NADH | - |
? | |
13-cis-retinal + NAD+ + H2O | type-2 isozyme, 2fold lower activity compared to all-trans-retinal | Mus musculus | 13-cis-retinoate + NADH | - |
? | |
9-cis-retinal + NAD+ + H2O | type-2 isozyme | Mus musculus | 9-cis-retinoate + NADH | - |
? | |
9-cis-retinal + NAD+ + H2O | type-2 isozyme, 5fold lower activity compared to all-trans-retinal | Mus musculus | 9-cis-retinoate + NADH | - |
? | |
all-trans-retinal + NAD+ + H2O | type-2 isozyme | Mus musculus | all-trans-retinoate + NADH | - |
? | |
all-trans-retinal + NAD+ + H2O | preferred substrate, type-2 isozyme | Mus musculus | all-trans-retinoate + NADH | - |
? | |
additional information | type-2 isozyme catalyzes the last step in biosynthesis of retinoic acid in embryos and adult reproductive organs, isozyme type-2 regulates the retinoic acid level during embryogenesis and tissue differentiation | Mus musculus | ? | - |
? | |
retinal + NAD+ + H2O | - |
Mus musculus | retinoate + NADH | - |
? | |
retinal + NAD+ + H2O | efficiency of conversion of all-trans-retinal to retinoic acid is 2fold and 5fold higher than 13-cis and 9-cis-retinal, respectively | Mus musculus | retinoate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Mus musculus |
? | x * 55000, recombinant detagged isozyme type-2, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
RALDH2 | - |
Mus musculus |
retinal dehydrogenase type-2 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
all retinal isomer substrates are converted to respective retinoic acids more efficiently compared to 37°C, might be due to instability of substrates at 37°C | Mus musculus |
25 | - |
assay at, recombinant isozyme type-2, probably higher activity than at 37°C due to higher stability of the substrates at 25°C | Mus musculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | lower activity at 37°C is also due to instability of the substrates | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
all-trans-retinal oxidation | Mus musculus |
9 | - |
recombinant isozyme type-2 | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus |