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Literature summary for 1.2.1.36 extracted from

  • Moffa, D.J.; Lotspeich, F.J.; Krause, R.F.
    Preparation and properties of retinal-oxidizing enzyme from rat intestinal mucosa (1970), J. Biol. Chem., 245, 439-447.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glutathione reduced form, reaction rate is maximal, with reduced glutathione Rattus norvegicus

General Stability

General Stability Organism
dialysis against distilled water, several hours, about 29% loss of activity Rattus norvegicus
quite unstable during extensive freezing and thawing Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-Iodoacetamide slight Rattus norvegicus
alpha,alpha'-dipyridyl slight Rattus norvegicus
Atabrine slight, FAD reverses inhibition Rattus norvegicus
FeCl3
-
Rattus norvegicus
NaCN slight Rattus norvegicus
NADH inhibits noncompetitively, inhibition not reversed by direct addition of NAD+ Rattus norvegicus
p-chloromercuribenzoate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
retinal
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ reaction rate is maximal with Fe2+ Rattus norvegicus
Iron metalloprotein, approximately 2 mol of iron per mol of enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
thin layer chromatography, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
retinal + NAD+ + H2O Rattus norvegicus acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal retinoate + NADH
-
ir
retinal + NAD+ + H2O Rattus norvegicus Wistar acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal retinoate + NADH
-
ir

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Wistar strain
-
Rattus norvegicus Wistar
-
Wistar strain
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
retinal + NAD+ + H2O = retinoate + NADH + H+ acts on both the all-trans- and 13-cis-form of retinal Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
intestinal mucosa
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
retinal + NAD+ + H2O acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal Rattus norvegicus retinoate + NADH
-
ir
retinal + NAD+ + H2O acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal Rattus norvegicus Wistar retinoate + NADH
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at 37°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
in 0.1 M sodium phosphate buffer, rather broad pH curve with pH-otimum at approximately pH 7.7 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD metalloflavoprotein, reaction rate is maximal with FAD Rattus norvegicus
NAD+ reaction rate is maximal with NAD+, stimulation by NAD+ reversed by direct addition of NADH Rattus norvegicus