General Stability | Organism |
---|---|
aldopantoate stabilizes against heat | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-4-dehydropantoate | - |
Pseudomonas sp. | |
NAD+ | above 0.3 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NAD+ | - |
Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-4-dehydropantoate + NAD+ | Pseudomonas sp. | enzyme required for degradation of pantothenate to alpha-ketoisovalerate | (R)-3,3-dimethylmalate + NADH | - |
? | |
(R)-4-dehydropantoate + NAD+ | Pseudomonas sp. P-2 | enzyme required for degradation of pantothenate to alpha-ketoisovalerate | (R)-3,3-dimethylmalate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
P-2 | - |
Pseudomonas sp. P-2 | - |
P-2 | - |
Purification (Comment) | Organism |
---|---|
P-2, partial | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
0°C, 0.1 M potassium phosphate pH 7.5, 0.015 M mercaptoethanol, 3 days | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-4-dehydropantoate + NAD+ | enzyme required for degradation of pantothenate to alpha-ketoisovalerate | Pseudomonas sp. | (R)-3,3-dimethylmalate + NADH | - |
? | |
(R)-4-dehydropantoate + NAD+ | enzyme required for degradation of pantothenate to alpha-ketoisovalerate | Pseudomonas sp. P-2 | (R)-3,3-dimethylmalate + NADH | - |
? | |
(R)-4-dehydropantoate + NAD+ + H2O | - |
Pseudomonas sp. | (R)-3,3-dimethylmalate + NADH | - |
? | |
(R)-4-dehydropantoate + NAD+ + H2O | - |
Pseudomonas sp. P-2 | (R)-3,3-dimethylmalate + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
4 min in presence of aldopantoate: no loss of activity, 1 min in absence of aldopantoate, complete loss of activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
or higher, activity increases from pH 7.0 to 9.5 | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | activity increases from pH 7.0 to 9.5 | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no diffusible cofactors found | Pseudomonas sp. |