Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.31 extracted from

  • Isobe, K.; Fukuda, N.; Nagasawa, S.
    Analysis of selective production of Nalpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde and Nalpha-benzyloxycarbonyl-L-aminoadipic acid by Rhodococcus sp. AIU Z-35-1 (2008), J. Biosci. Bioeng., 105, 152-156.
    View publication on PubMed
Search for more literature for 1.2.1.31

Inhibitors

Inhibitors Comment Organism Structure
additional information dehydrogenase activity is not inhibited by Nalpha-Z-L-lysine and Nalpha-benzyloxycarbonyl-L-aminoadipate at pH 5.0 or 7.0 Rhodococcus sp. (in: high G+C Gram-positive bacteria)
Nalpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.888
-
 Nalpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde at pH 5.0 Rhodococcus sp. (in: high G+C Gram-positive bacteria)
1.34
-
 Nalpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde at pH 7.0 Rhodococcus sp. (in: high G+C Gram-positive bacteria)

Organism

Organism UniProt Comment Textmining
Rhodococcus sp. (in: high G+C Gram-positive bacteria)
-
-
-
Rhodococcus sp. (in: high G+C Gram-positive bacteria) AIU Z-35-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.44
-
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde + NAD+ + H2O
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria) Nalpha-benzyloxycarbonyl-L-aminoadipate + NADH + H+
-
 ?
Nalpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde + NAD+ + H2O
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria) AIU Z-35-1 Nalpha-benzyloxycarbonyl-L-aminoadipate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
Nalpha-Z-L-AASA dehydrogenase
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 enzyme activity at 10°C is less than 10% of the maximum activity Rhodococcus sp. (in: high G+C Gram-positive bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 Nalpha-benzyloxycarbonyl-L-aminoadipate production by cell reaction is optimum at pH 7.0, although the enzyme exhibits optimum activity at pH 8.0 Rhodococcus sp. (in: high G+C Gram-positive bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 activity is significantly decreased in acidic pH regions, yet the difference in activity is maximum at pH 5.0. The dehydrogenase is unstable in alkaline pH regions Rhodococcus sp. (in: high G+C Gram-positive bacteria)

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
8.6
-
 Nalpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde at pH 7.0 Rhodococcus sp. (in: high G+C Gram-positive bacteria)
50
-
 Nalpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde at pH 5.0 Rhodococcus sp. (in: high G+C Gram-positive bacteria)