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Literature summary for 1.2.1.31 extracted from

  • Hijarrubia, M.J.; Aparicio, J.F.; Martin, J.F.
    Domain structure characterization of the multifunctional alpha-aminoadipate reductase from Penicillium chrysogenum by limited proteolysis. Activation of alpha-aminoadipate does not require the peptidyl carrier protein box or the reduction domain (2003), J. Biol. Chem., 278, 8250-8256.
    View publication on PubMed
Search for more literature for 1.2.1.31

Activating Compound

Activating Compound Comment Organism Structure
ATP presence of ATP required Penicillium chrysogenum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 75% inhibition Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 NADPH pH 8, 30°C Penicillium chrysogenum
1.3
-
 ATP pH 8, 30°C Penicillium chrysogenum
1.4
-
 L-2-aminoadipate pH 8, 30°C Penicillium chrysogenum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolue requiremnt, optimal concentration: 10 mM Penicillium chrysogenum
Mn2+ optimal concentration: 5 mM, 60% of the maximal activity attained with Mg2+ Penicillium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
155000
-
 1 * 155000, SDS-PAGE Penicillium chrysogenum
171000
-
 gel filtration Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium chrysogenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Penicillium chrysogenum

Storage Stability

Storage Stability Organism
-20°C, 50% v/v glycerol, rapid loss of activity Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminoadipate + NADPH + H+ presence of ATP required Penicillium chrysogenum L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
 ?
S-carboxymethyl-L-cysteine + NADPH 5% of the activity with L-2-aminoadipate Penicillium chrysogenum ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 155000, SDS-PAGE Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 29
-
 Penicillium chrysogenum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 1 h, stable Penicillium chrysogenum
30
-
 1 h, 20% loss of activity Penicillium chrysogenum
37
-
 15 min, 50% loss of activity Penicillium chrysogenum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
66
-
 L-2-aminoadipate pH 8, 30°C Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Penicillium chrysogenum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Penicillium chrysogenum