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Literature summary for 1.2.1.31 extracted from

  • Ehmann, D.E.; Gehring, A.M.; Walsh, C.T.
    Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5 (1999), Biochemistry, 38, 6171-6177.
    View publication on PubMed
Search for more literature for 1.2.1.31

Cloned(Commentary)

Cloned (Comment) Organism
Lys5 and Lys2 cloned from Saccharomyces cerevisiae DNA, overexpressed in Escherichia coli, and purified, Lys5/Lys2 pair is a two component system in which Lys5 covalently primes Lys2, allowing alpha-aminoadipate reductase activity by holo-Lys2 with catalytic cycles of autoaminoacylation and reductive clevage Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.62
-
 NADPH of Lys2 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 Lys5 from induced E. coli cells Saccharomyces cerevisiae
155000
-
 full-length Lys2 from induced E. coli cells Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alpha-aminoadipate + NAD(P)H Saccharomyces cerevisiae
-
 L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information Lys5 demonstrates phosphopantetheinyl transferase activity toward the 14-kDa Lys2 peptidyl carrier protein fragment, with a Km of 0.001 mM and a turnover number of 3/min Saccharomyces cerevisiae
side-chain modification Lys5 is a specific posttranslational modification catalyst, using coenzyme A as a cosubstrate to phosphopantetheinylate Ser880 of the Lys2 activating it for catalysis Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-aminoadipic semialdehyde + NAD+ + H2O
-
 Saccharomyces cerevisiae alpha-aminoadipic acid + NADH + H+
-
 ?
L-2-aminoadipate + NADPH
-
 Saccharomyces cerevisiae L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
 ?
L-alpha-aminoadipate + NAD(P)H
-
 Saccharomyces cerevisiae L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.2
-
 alpha-aminoadipate semialdehyde production from holo-lys2 Saccharomyces cerevisiae