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Literature summary for 1.2.1.31 extracted from

  • Tsai, C.H.; Henderson, L.M.
    Degradation of O-phosphohydroxylysine in rat liver. Purification and properties of 2-aminoadipic semialdehyde dehydrogenase (1974), J. Biol. Chem., 249, 5790-5792.
    View publication on PubMed
Search for more literature for 1.2.1.31

General Stability

General Stability Organism
freezing-thawing, frozen enzyme loses 10-20% of activity during each freeze-thaw cycle Rattus norvegicus
lyophilization inactivates Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2-aminoadipic semialdehyde
-
 Rattus norvegicus
0.2
-
 alpha-aminoadipate NAD+ Rattus norvegicus
0.2
-
 NAD+
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-aminoadipate-semialdehyde + NAD+ Rattus norvegicus mammalian degradation of hydroxy-L-lysine via phosphohydrolysine and 2-aminoadipic semialdehyde to 2-aminoadipic acid L-2-aminoadipate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation and column chromatography on CM-Sephadex Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.185
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
0°C, 50% loss of activity, 24 h Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-aminoadipic semialdehyde + NAD+ + H2O
-
 Rattus norvegicus alpha-aminoadipic acid + NADH + H+
-
 ?
L-2-aminoadipate 6-semialdehyde + NAD+ + H2O
-
 Rattus norvegicus L-2-aminoadipate + NADH
-
 ?
L-2-aminoadipate-semialdehyde + NAD+ mammalian degradation of hydroxy-L-lysine via phosphohydrolysine and 2-aminoadipic semialdehyde to 2-aminoadipic acid Rattus norvegicus L-2-aminoadipate + NADH
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5: about 30% of activity maximum, pH 9: about 40% of activity maximum Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rattus norvegicus