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Literature summary for 1.2.1.31 extracted from

  • Schmidt, H.; Bode, R.; Lindner, M.; Birnbaum, D.
    Lysine biosynthesis in the yeast Candida maltosa: properties of some enzymes and regulation of the biosynthetic pathway (1985), J. Basic Microbiol., 25, 675-681.
No PubMed abstract available
Search for more literature for 1.2.1.31

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Candida maltosa
Fe2+
-
 Candida maltosa
glutamate competitive inhibitor with respect to alpha-aminoadipate Candida maltosa
Hg2+
-
 Candida maltosa
L-aspartate competitive inhibitor with respect to alpha-aminoadipate Candida maltosa
Sn2+
-
 Candida maltosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 NADPH
-
 Candida maltosa
0.2
-
 alpha-aminoadipate
-
 Candida maltosa
0.38
-
 NADH
-
 Candida maltosa
0.5
-
 ATP
-
 Candida maltosa
3.8
-
 MgCl2
-
 Candida maltosa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates Candida maltosa
Cu2+ inhibits Candida maltosa
Mg2+ stimulates Candida maltosa
Mg2+ required for activity Candida maltosa
Mn2+ stimulates Candida maltosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Candida maltosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alpha-aminoadipate + NAD(P)H Candida maltosa in presence of ATP and Mg2+ L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?
L-alpha-aminoadipate + NAD(P)H Candida maltosa involved in lysine biosynthetic pathway L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?
L-alpha-aminoadipate + NAD(P)H Candida maltosa L4 in presence of ATP and Mg2+ L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?
L-alpha-aminoadipate + NAD(P)H Candida maltosa L4 involved in lysine biosynthetic pathway L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Candida maltosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using column chromatography on Sephadex G-25 and Sephadex G-200 Candida maltosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0063
-
 5 mM L-lysine as supplement during growth of the enzyme Candida maltosa
0.0072
-
 no supplement added during growth of the enzyme Candida maltosa
0.0138
-
 10 mM amitrole as supplement during growth of the enzyme Candida maltosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-aminoadipic semialdehyde + NAD+ + H2O
-
 Candida maltosa alpha-aminoadipic acid + NADH + H+
-
 ?
alpha-aminoadipic semialdehyde + NAD+ + H2O
-
 Candida maltosa L4 alpha-aminoadipic acid + NADH + H+
-
 ?
L-2-aminoadipate + NADPH in presence of ATP + Mg2+ Candida maltosa L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
 ?
L-2-aminoadipate + NADPH in presence of ATP + Mg2+ Candida maltosa L4 L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
 ?
L-alpha-aminoadipate + NAD(P)H in presence of ATP and Mg2+ Candida maltosa L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?
L-alpha-aminoadipate + NAD(P)H involved in lysine biosynthetic pathway Candida maltosa L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?
L-alpha-aminoadipate + NAD(P)H in presence of ATP and Mg2+ Candida maltosa L4 L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?
L-alpha-aminoadipate + NAD(P)H involved in lysine biosynthetic pathway Candida maltosa L4 L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Candida maltosa

Cofactor

Cofactor Comment Organism Structure
NADH can replace NADPH with lower activity and higher Km Candida maltosa
NADPH
-
 Candida maltosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5
-
 L-glutamate
-
 Candida maltosa
4.2
-
 L-aspartate
-
 Candida maltosa