Cloned (Comment) | Organism |
---|---|
- |
Nocardia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
128300 | - |
x * 128300, deduced from gene sequence, x * 132400, SDS-PAGE, recombinant enzyme carrying His-tag | Nocardia sp. |
132400 | - |
x * 128300, deduced from gene sequence, x * 132400, SDS-PAGE, recombinant enzyme carrying His-tag | Nocardia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia sp. | Q6RKB1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme with his-tag | Nocardia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
pH 7.5, 25°C, recombinant enzyme with His-tag | Nocardia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoate + NADPH + ATP | best substrate | Nocardia sp. | benzaldehyde + NADP+ + AMP + phosphate | - |
? | |
coniferic acid + NADPH + ATP | - |
Nocardia sp. | coniferyl aldehyde + NADP+ + AMP + phosphate | - |
? | |
vanillic acid + NADPH + H+ + ATP | - |
Nocardia sp. | vanillin + NADP+ + AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 128300, deduced from gene sequence, x * 132400, SDS-PAGE, recombinant enzyme carrying His-tag | Nocardia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADH | Nocardia sp. | |
NADPH | - |
Nocardia sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Nocardia sp. | calculated from amino acid sequence | - |
4.9 |