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Literature summary for 1.2.1.3 extracted from

  • Tuck, L.R.; Altenbach, K.; Ang, T.F.; Crawshaw, A.D.; Campopiano, D.J.; Clarke, D.J.; Marles-Wright, J.
    Insight into coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans (2016), Sci. Rep., 6, 22108.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lachnoclostridium phytofermentans

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NAD+, sitting drop vapor diffusion method, using 0.1 M sodium acetate pH 4.7, 1.8 M ammonium sulfate Lachnoclostridium phytofermentans

Inhibitors

Inhibitors Comment Organism Structure
Butyraldehyde
-
 Lachnoclostridium phytofermentans
hexanaldehyde
-
 Lachnoclostridium phytofermentans
pentanaldehyde
-
 Lachnoclostridium phytofermentans
propionaldehyde
-
 Lachnoclostridium phytofermentans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.82
-
 propionaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.74
-
 Butyraldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
2.2
-
 pentanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
2.9
-
 hexanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
5.16
-
 acetaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
191300
-
 native mass spectrometry Lachnoclostridium phytofermentans

Organism

Organism UniProt Comment Textmining
Lachnoclostridium phytofermentans A9KN57
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, Q-sepharose column chromatography, and S200 gel filtration Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans acetate + NADH + H+
-
 ?
butyraldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans butyrate + NADH + H+
-
 ?
hexanaldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans hexanoate + NADH + H+
-
 ?
pentanaldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans pentanoate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans propanoate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Lachnoclostridium phytofermentans

Synonyms

Synonyms Comment Organism
ALDDH
-
 Lachnoclostridium phytofermentans
Cphy_1178 locus name Lachnoclostridium phytofermentans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.62
-
 acetaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
3.44
-
 Butyraldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
3.45
-
 propionaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
5.44
-
 pentanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
5.61
-
 hexanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Lachnoclostridium phytofermentans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
12.61
-
 pentanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
17.31
-
 propionaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
23.52
-
 hexanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
144.3
-
 Butyraldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.31
-
 acetaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.93
-
 hexanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.98
-
 Butyraldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
2.47
-
 pentanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
4.21
-
 propionaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans