Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
E149D | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149D/I200G | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149D/I200V | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149N | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149N/I200G | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149N/I200V | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149Q | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149Q/I200G | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149Q/I200V | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149T | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149T/I200G | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
E149T/I200V | the mutant shows a good catalysis with NADP+ compared to the wild type enzyme | Arabidopsis thaliana |
E149T/V178R/I200V | the mutant uses NADP+ with almost 7fold higher catalytic efficiency compared to NAD+ | Arabidopsis thaliana |
I200G | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
I200V | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
trans-2-nonenal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.008 | - |
Nonanal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.0322 | - |
trans-2-nonenal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.065 | - |
Nonanal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.071 | - |
hexanal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.072 | - |
hexanal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.18 | - |
trans-2-hexenal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.199 | - |
trans-2-hexenal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.421 | - |
NAD+ | wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.441 | - |
NAD+ | mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.496 | - |
NAD+ | mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.564 | - |
NAD+ | mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.63 | - |
NAD+ | mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.886 | - |
NAD+ | mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.991 | - |
NAD+ | mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
0.992 | - |
NAD+ | mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
1.068 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
1.092 | - |
NAD+ | mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
1.262 | - |
NAD+ | mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
1.622 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
1.648 | - |
NAD+ | mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
1.649 | - |
NAD+ | mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
1.829 | - |
NAD+ | mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
1.856 | - |
NAD+ | mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
2.115 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
2.524 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
3.218 | - |
NAD+ | mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
3.418 | - |
NAD+ | mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
3.651 | - |
NAD+ | mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
4.059 | - |
NAD+ | mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
4.136 | - |
NAD+ | mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q70DU8 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography, and Superdex 200 gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexanal + NAD+ + H2O | - |
Arabidopsis thaliana | hexanoate + NADH + H+ | - |
? | |
additional information | no activity with NADP+ | Arabidopsis thaliana | ? | - |
? | |
nonanal + NAD+ + H2O | - |
Arabidopsis thaliana | nonanoate + NADH + H+ | - |
? | |
trans-2-hexenal + NAD+ + H2O | - |
Arabidopsis thaliana | (E)-hex-2-enoate + NADH + H+ | - |
? | |
trans-2-nonenal + NAD+ + H2O | - |
Arabidopsis thaliana | (E)-non-2-enoate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALDH3H1 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
trans-2-hexenal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
1.8 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
2.2 | - |
trans-2-hexenal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
2.2 | - |
NAD+ | mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
2.7 | - |
trans-2-nonenal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
3.1 | - |
NAD+ | mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
3.8 | - |
trans-2-nonenal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
4 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
8.2 | - |
hexanal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
10.2 | - |
NAD+ | mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
10.7 | - |
NAD+ | mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
11.2 | - |
hexanal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
11.4 | - |
NAD+ | mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
12.2 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
12.4 | - |
NAD+ | mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
12.7 | - |
NAD+ | mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
12.7 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
13 | - |
Nonanal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
13.1 | - |
NAD+ | mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
15.7 | - |
NAD+ | mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
16.3 | - |
NAD+ | mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
16.6 | - |
NAD+ | mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
17.2 | - |
NAD+ | wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
17.9 | - |
Nonanal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
18 | - |
NAD+ | mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
18.5 | - |
NAD+ | mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
20.1 | - |
NAD+ | mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
23.2 | - |
NAD+ | mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
26.9 | - |
NAD+ | mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
33.9 | - |
NAD+ | mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
2.8 | - |
NAD+ | mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
3.8 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
4 | - |
NAD+ | mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
4.8 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
5 | - |
NAD+ | mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
5.1 | - |
NAD+ | mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
5.3 | - |
trans-2-hexenal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
6 | - |
NAD+ | mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
6.1 | - |
NAD+ | mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
6.4 | - |
NAD+ | mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
6.5 | - |
NAD+ | mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
8.1 | - |
NAD+ | mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
9.5 | - |
NAD+ | mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
9.9 | - |
NAD+ | mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
9.9 | - |
NAD+ | mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
12.4 | - |
trans-2-hexenal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
12.5 | - |
NAD+ | mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
13.2 | - |
NAD+ | mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
14.3 | - |
NAD+ | mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
24.3 | - |
NAD+ | mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
26.3 | - |
NAD+ | mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
37.3 | - |
NAD+ | mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
40.9 | - |
NAD+ | wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C | Arabidopsis thaliana | |
115 | - |
hexanal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
120 | - |
trans-2-nonenal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
154 | - |
hexanal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
213 | - |
Nonanal | mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C | Arabidopsis thaliana | |
918 | - |
trans-2-nonenal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana | |
2166 | - |
Nonanal | wild type enzyme, at pH 8.0 and 25°C | Arabidopsis thaliana |