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Literature summary for 1.2.1.3 extracted from

  • Yoval-Sanchez, B.; Pardo, J.P.; Rodriguez-Zavala, J.S.
    New insights into the half-of-the-sites reactivity of human aldehyde dehydrogenase 1A1 (2013), Proteins, 81, 1330-1339.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the GFP-tagged enzyme is expressed in Escherichia coli BL21(DE3) pLysS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D80G/S82A the unstable mutant starts to denature at urea concentrations below 0.5 M, reaching half of the denaturation at 1.2 M urea Homo sapiens

General Stability

General Stability Organism
urea concentrations higher than 4.5 M almost completely denature wild type isoform ALDH1A1 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acrolein
-
Homo sapiens
arsenite 47% residual activity at 1 mM arsenite at pH 9.5, 28% residual activity at 2 mM arsenite at pH 7.4 Homo sapiens
Chloroacetaldehyde 86% residual activity at 1 mM chloroacetaldehyde at pH 9.5 Homo sapiens
Mg2+ wild type enzyme activity is inhibited (64%) by Mg2+ at pH 7.4, whereas the GFP-tagged enzyme activity is not affected by Mg2+ Homo sapiens
additional information not influenced by acetaldehyde Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
malondialdehyde wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
0.009
-
acrolein wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
0.011
-
NAD+ wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
0.012
-
propionaldehyde wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
0.136
-
NAD+ mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
0.189
-
propionaldehyde mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
0.595
-
glyceraldehyde wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
4 * 50000, SDS-PAGE Homo sapiens
85000
-
2 * 85000, GFP-tagged enzyme, SDS-PAGE Homo sapiens
170000
-
GFP-tagged enzyme, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel chelating Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrolein + NAD+ + H2O
-
Homo sapiens ? + NADH + H+
-
?
glyceraldehyde + NAD+ + H2O
-
Homo sapiens glycerate + NADH + H+
-
?
malondialdehyde + NAD+ + H2O
-
Homo sapiens ? + NADH + H+
-
?
additional information no activity with aromatic aldehydes such as benzaldehyde, nitrobenzaldehyde, methoxybenzaldehyde, or phenylacetaldehyde Homo sapiens ?
-
?
propionaldehyde + NAD+ + H2O
-
Homo sapiens propionate + NADH + H+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 85000, GFP-tagged enzyme, SDS-PAGE Homo sapiens
homotetramer 4 * 50000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase 1A1 isoform Homo sapiens
ALDH1A1 isoform Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens