Cloned (Comment) | Organism |
---|---|
the GFP-tagged enzyme is expressed in Escherichia coli BL21(DE3) pLysS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D80G/S82A | the unstable mutant starts to denature at urea concentrations below 0.5 M, reaching half of the denaturation at 1.2 M urea | Homo sapiens |
General Stability | Organism |
---|---|
urea concentrations higher than 4.5 M almost completely denature wild type isoform ALDH1A1 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acrolein | - |
Homo sapiens | |
arsenite | 47% residual activity at 1 mM arsenite at pH 9.5, 28% residual activity at 2 mM arsenite at pH 7.4 | Homo sapiens | |
Chloroacetaldehyde | 86% residual activity at 1 mM chloroacetaldehyde at pH 9.5 | Homo sapiens | |
Mg2+ | wild type enzyme activity is inhibited (64%) by Mg2+ at pH 7.4, whereas the GFP-tagged enzyme activity is not affected by Mg2+ | Homo sapiens | |
additional information | not influenced by acetaldehyde | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
malondialdehyde | wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C | Homo sapiens | |
0.009 | - |
acrolein | wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C | Homo sapiens | |
0.011 | - |
NAD+ | wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C | Homo sapiens | |
0.012 | - |
propionaldehyde | wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C | Homo sapiens | |
0.136 | - |
NAD+ | mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C | Homo sapiens | |
0.189 | - |
propionaldehyde | mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C | Homo sapiens | |
0.595 | - |
glyceraldehyde | wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, SDS-PAGE | Homo sapiens |
85000 | - |
2 * 85000, GFP-tagged enzyme, SDS-PAGE | Homo sapiens |
170000 | - |
GFP-tagged enzyme, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel chelating Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrolein + NAD+ + H2O | - |
Homo sapiens | ? + NADH + H+ | - |
? | |
glyceraldehyde + NAD+ + H2O | - |
Homo sapiens | glycerate + NADH + H+ | - |
? | |
malondialdehyde + NAD+ + H2O | - |
Homo sapiens | ? + NADH + H+ | - |
? | |
additional information | no activity with aromatic aldehydes such as benzaldehyde, nitrobenzaldehyde, methoxybenzaldehyde, or phenylacetaldehyde | Homo sapiens | ? | - |
? | |
propionaldehyde + NAD+ + H2O | - |
Homo sapiens | propionate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 85000, GFP-tagged enzyme, SDS-PAGE | Homo sapiens |
homotetramer | 4 * 50000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase 1A1 | isoform | Homo sapiens |
ALDH1A1 | isoform | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |