Protein Variants | Comment | Organism |
---|---|---|
C302S | the mutation leads to more than 90% loss of all enzyme activities (0.29% residual dehydrogenase activity at pH 9.0 and 0.32% residual dehydrogenase activity at pH 7.5 compared to the wild type enzyme) | Homo sapiens |
E268Q | the mutant shows 1.39% residual dehydrogenase activity at pH 9.0 and 0.88% residual dehydrogenase activity at pH 7.5 compared to the wild type enzyme. The mutant exhibits virtually unaffected rates of nitroglycerin denitration despite low dehydrogenase and esterase activities. The mutant exhibits about 50% lower rates of superoxide formation than the wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Chloral hydrate | - |
Homo sapiens | |
nitroglycerin | complete inhibition at 0.05 mM nitroglycerin which can be reversed by the addition of 0.4 mM dithiothreitol | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
4 * 54000 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P05091 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Homo sapiens | formate + NADH + H+ | - |
? | |
additional information | ALDH2 denitrates nitroglycerin to 1,2-glyceryl dinitrate and nitrite but also catalyzes reduction of nitroglycerin to NO. In addition to aldehyde oxidation, ALDH2 catalyzes ester hydrolysis. The esterase activity is stimulated by NAD+, but the cofactor is not essential for the reaction | Homo sapiens | ? | - |
? | |
additional information | the enzyme shows esterase activity with 4-nitrophenyl acetate | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 54000 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase-2 | - |
Homo sapiens |
ALDH2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | ALDH2 plays an essential role in nitroglycerin bioactivation. ALDH2-catalyzed superoxide formation may essentially contribute to oxidative stress in nitroglycerin-exposed blood vessels | Homo sapiens |