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Literature summary for 1.2.1.3 extracted from

  • Glatt, H.; Rost, K.; Frank, H.; Seidel, A.; Kollock, R.
    Detoxification of promutagenic aldehydes derived from methylpyrenes by human aldehyde dehydrogenases ALDH2 and ALDH3A1 (2008), Arch. Biochem. Biophys., 477, 196-205.
    View publication on PubMed
Search for more literature for 1.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens
expressed in Escherichia coli XL-1 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000027
-
 1-formyl-8-methylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
0.00004
-
 4-formylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
0.00015
-
 1-formyl-6-methylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
0.00038
-
 2-formylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
0.00048
-
 acetaldehyde ALDH2, at pH 7.5 and 37°C Homo sapiens
0.00078
-
 4-formylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
0.0009
-
 1-formylpyrene ALDH2, at pH 7.5 and 37°C Homo sapiens
0.0016
-
 1-formyl-6-methylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
0.004
-
 1-formylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
0.004
-
 1-formyl-8-methylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
0.011
-
 2-formylpyrene in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
0.23
-
 benzaldehyde in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
0.42
-
 formaldehyde ALDH2, at pH 7.5 and 37°C Homo sapiens
12
-
 propionaldehyde in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens
80
-
 acetaldehyde in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50390
-
 calculated from sequence of cDNA Homo sapiens
54000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens P05091
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE MacroPrep column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
buccal mucosa
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-formyl-6-methylpyrene + NAD+
-
 Homo sapiens 6-methylpyrene 1-carboxylate + NADH + H+
-
 ?
1-formyl-6-methylpyrene + NAD+ + H2O
-
 Homo sapiens 6-methylpyrene-1-carboxylic acid + NADH + H+
-
 ?
1-formyl-8-methylpyrene + NAD+ + H2O
-
 Homo sapiens 8-methylpyrene-1-carboxylic acid + NADH + H+
-
 ?
1-formyl-8-methylpyrene + NADP+
-
 Homo sapiens 8-methylpyrene 1-carboxylate + NADPH + H+
-
 ?
1-formylpyrene + NAD+
-
 Homo sapiens pyrene 1-carboxylate + NADH + H+
-
 ?
1-formylpyrene + NAD+ + H2O
-
 Homo sapiens pyrene-1-carboxylic acid + NADH + H+
-
 ?
2-formylpyrene + NAD+
-
 Homo sapiens pyrene 2-carboxylate + NADH + H+
-
 ?
2-formylpyrene + NAD+ + H2O
-
 Homo sapiens pyrene-2-carboxylic acid + NADH + H+
-
 ?
4-formylpyrene + NAD+
-
 Homo sapiens pyrene 4-carboxylate + NADH + H+
-
 ?
4-formylpyrene + NAD+ + H2O
-
 Homo sapiens pyrene-4-carboxylic acid + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Homo sapiens benzoate + NADH + H+
-
 ?
formaldehyde + NAD+
-
 Homo sapiens formate + NADH + H+
-
 ?
formaldehyde + NAD+ + H2O
-
 Homo sapiens formate + NADH + H+
-
 ?
additional information no activity with formaldehyde Homo sapiens ?
-
 ?
propionaldehyde + NAD+
-
 Homo sapiens propionate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O
-
 Homo sapiens propionate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
ALDH2
-
 Homo sapiens
ALDH3A1
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Homo sapiens
9.5
-
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
 the activity at a physiological pH value of 7.4 is nearly 50% of the activity observed at the optimal pH value Homo sapiens
7.4
-
 the activity at a physiological pH value (7.4) is nearly 40% of the activity observed at the optimal pH value Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens