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Literature summary for 1.2.1.3 extracted from

  • Lindahl, R.; Evces, S.
    Rat liver aldehyde dehydrogenase. I. Isolation and characterization of four high Km normal liver isozymes (1984), J. Biol. Chem., 259, 11986-11990.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Chloral hydrate inhibition of isoenzyme I and II from mitochondria and isoenzyme II from microsomes, no inhibition of isoenzyme I from microsomes Rattus norvegicus
Disulfiram inhibits activity of mitochondrial isoenzyme I with NAD+ and activity of isoenzyme I and II from microsomes and mitochondria with NADP+ Rattus norvegicus
NAD+ 0.1 mM, significant inhibition of esterase activity Rattus norvegicus
p-hydroxymercuribenzoate 0.05 mM, potent inhibition of isoenzyme I from microsomes and mitochondria, isoenzyme II from microsomes and mitochondria is less sensitive Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
NAD+ reaction with propanal, enzyme II from mitochondria Rattus norvegicus
0.036
-
NAD+ reaction with propanal, enzyme I from mitochondria Rattus norvegicus
0.095
-
NAD+ reaction with propanal, enzyme II from microsomes Rattus norvegicus
0.097
-
NAD+ reaction with propanal, enzyme I from microsomes Rattus norvegicus
0.15
-
propanal enzyme I and II from mitochondria Rattus norvegicus
0.27
-
NADP+ reaction with benzaldehyde, enzyme II from microsomes Rattus norvegicus
0.44
-
NADP+ reaction with benzaldehyde, enzyme II from mitochondria Rattus norvegicus
0.44
-
NADP+ reaction with benzaldehyde, enzyme I from microsomes Rattus norvegicus
0.91
-
NADP+ reaction with benzaldehyde, enzyme I from mitochondria Rattus norvegicus
1.6
-
benzaldehyde enzyme I from mitochondria Rattus norvegicus
1.7
-
propanal enzyme II from microsome Rattus norvegicus
2.4
-
acetaldehyde enzyme I from microsome Rattus norvegicus
3
-
benzaldehyde enzyme II from microsomes Rattus norvegicus
5.9
-
benzaldehyde enzyme I from microsomes Rattus norvegicus
50
-
benzaldehyde enzyme II from mitochondria Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome isoenzyme I and II Rattus norvegicus
-
-
mitochondrion isoenzyme I and II Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 0.5 mM, slight activation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Rattus norvegicus
170000
-
mitochondrial isoenzyme I, gel filtration Rattus norvegicus
250000
-
mitochondrial isoenzyme II, microsomal isoenzyme I and II, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-20°C, all isoenzymes are stable for up to 1 month Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Rattus norvegicus acetate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O
-
Rattus norvegicus benzoate + NADH + H+
-
?
benzaldehyde + NADP+
-
Rattus norvegicus benzoate + NADPH
-
?
p-nitrophenyl acetate + H2O esterase activity of the various isoenzymes is less than 7% of the activity with propanal and NAD+ Rattus norvegicus p-nitrophenol + acetate
-
?
phenylacetaldehyde + NAD+ + H2O
-
Rattus norvegicus phenylacetate + NADH + H+
-
?
propanal + NAD+ + H2O
-
Rattus norvegicus propionate + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
5 min, microsomal isoenzymes and mitochondrial isoenzyme I, more than 90% loss of activity. Mitochondrial isoenzyme II retains 20% of its original activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
microsomal isoenzyme I and II and mitochondrial isoenzymes with NADP+ Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+ all isoenzymes prefer NAD+ over NADP+ Rattus norvegicus
NADP+ all isoenzymes prefer NAD+ over NADP+ Rattus norvegicus