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Literature summary for 1.2.1.3 extracted from

  • Rout, U.K.; Weiner, H.
    Involvement of Serine 74 in the Enzyme-Coenzyme Interaction of Rat Liver Mitochondrial Aldehyde Dehydrogenase (1994), Biochemistry, 33, 8955-8961.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
S74A half-life of the mutant enzyme at 50°C is 1 min compared to the half-life of the native enzyme of 1 min. Mutation diminishes NAD+ binding, affecting both the on and the off rates, as well as the rate-limiting step. About 100fold higher Km-value for NAD+ Rattus norvegicus
S74C about 70fold higher Km-value for NAD+ Rattus norvegicus
S74T about 100fold higher Km-value for NAD+ Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ADPribose competitive Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
benzaldehyde native enzyme Rattus norvegicus
0.0001
-
p-nitrobenzaldehyde native enzyme Rattus norvegicus
0.0001
-
p-Methoxybenzaldehyde native enzyme Rattus norvegicus
0.0001
-
4-(dimethylamino)cinnamaldehyde native enzyme Rattus norvegicus
0.0002
-
benzaldehyde mutant enzyme S74A Rattus norvegicus
0.0003
-
4-(dimethylamino)cinnamaldehyde mutant enzyme S74A Rattus norvegicus
0.0004
-
acetaldehyde native enzyme Rattus norvegicus
0.0004
-
p-nitrobenzaldehyde mutant enzyme S74A Rattus norvegicus
0.0004
-
p-Methoxybenzaldehyde mutant enzyme S74A Rattus norvegicus
0.0007
-
propanal native enzyme Rattus norvegicus
0.0007
-
Butanal native enzyme Rattus norvegicus
0.0008
-
hexanal native enzyme Rattus norvegicus
0.0008
-
propanal native enzyme and mutant enzyme S74A Rattus norvegicus
0.0009
-
Glutaraldehyde native enzyme Rattus norvegicus
0.0009
-
propanal mutant enzyme S74A Rattus norvegicus
0.002
-
Butanal mutant enzyme S74A Rattus norvegicus
0.0024
-
hexanal mutant enzyme S74A Rattus norvegicus
0.0027
-
acetaldehyde mutant enzyme S74A Rattus norvegicus
0.003
-
Glutaraldehyde mutant enzyme S74A Rattus norvegicus
0.01
-
NAD+ native enzyme, reaction with propanal Rattus norvegicus
0.038
-
glycolaldehyde native enzyme Rattus norvegicus
0.077
-
glycolaldehyde mutant enzyme S74A Rattus norvegicus
0.178
-
formaldehyde native enzyme Rattus norvegicus
0.7
-
formaldehyde mutant enzyme S74A Rattus norvegicus
1.9
-
NAD+ mutant enzyme S74A, reaction with propanal Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and mutant enzyme S74A Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Rattus norvegicus acetate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O
-
Rattus norvegicus benzoate + NADH + H+
-
?
butanal + NAD+ + H2O
-
Rattus norvegicus butyrate + NADH + H+
-
?
formaldehyde + NAD+ + H2O
-
Rattus norvegicus formate + NADH
-
?
glutaraldehyde + NAD+ + H2O
-
Rattus norvegicus glutarate + NADH
-
?
glycolaldehyde + NAD+ + H2O
-
Rattus norvegicus glycolate + NADH
-
?
hexanal + NAD+ + H2O
-
Rattus norvegicus hexanoate + NADH + H+
-
?
m-methoxybenzaldehyde + NAD+ + H2O
-
Rattus norvegicus m-methoxybenzoate + NADH + H+
-
?
p-(dimethylamino)cinnamaldehyde + NAD+ + H2O
-
Rattus norvegicus p-(dimethylamino)cinnamate + NADH
-
?
p-nitrobenzaldehyde + NAD+ + H2O
-
Rattus norvegicus p-nitrobenzoate + NADH
-
?
propanal + NAD+ + H2O
-
Rattus norvegicus propionate + NADH
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
mutant enzyme S74A and native enzyme, stable Rattus norvegicus
50
-
half-life of mutant enzyme S74A is 1 min, half-life of native enzyme is 4 min Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.32
-
ADPribose native enzyme Rattus norvegicus
6.8
-
ADPribose mutant enzyme S74A Rattus norvegicus