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Literature summary for 1.2.1.29 extracted from

  • Nakamura, T.; Ichinose, H.; Wariishi, H.
    Cloning and heterologous expression of two aryl-aldehyde dehydrogenases from the white-rot basidiomycete Phanerochaete chrysosporium (2010), Biochem. Biophys. Res. Commun., 394, 470-475.
    View publication on PubMed
Search for more literature for 1.2.1.29

Cloned(Commentary)

Cloned (Comment) Organism
PcALDH1 and PcALDH2 expressed as C-terminal histidine-tagged proteins in Escherichia coli cells Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53300
-
 PcALDH1, calculated from sequence Phanerodontia chrysosporium
54400
-
 PcALDH2, calculated from sequence Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
 white-rot basidiomycete
-

Purification (Commentary)

Purification (Comment) Organism
PcALDH1 and PcALDH2 recombinant protein purified with a His-tag affinity column Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 3,4,5-trimethoxybenzoic acid + NADH + H+
-
 ?
3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium ATCC 34541 3,4,5-trimethoxybenzoic acid + NADH + H+
-
 ?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 3,4-dihydroxybenzoic acid + NADH + H+
-
 ?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium ATCC 34541 3,4-dihydroxybenzoic acid + NADH + H+
-
 ?
3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
 ?
3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium ATCC 34541 3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
 ?
3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 3,4-dimethoxybenzoic acid + NADH + H+
-
 ?
3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium ATCC 34541 3,4-dimethoxybenzoic acid + NADH + H+
-
 ?
4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
 ?
4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium ATCC 34541 4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
 ?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O 4-hydroxy-3-methoxybenzaldehyde i.e. vanillin Phanerodontia chrysosporium 4-hydroxy-3-methoxybenzoic acid + NADH + H+
-
 ?
4-hydroxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 4-hydroxybenzoic acid + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium benzoic acid + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
aryl-aldehyde dehydrogenase
-
 Phanerodontia chrysosporium
PcALDH1
-
 Phanerodontia chrysosporium
PcALDH2
-
 Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
4 11 enzymatic reactions carried out at pH 4.0 show only 5–10% of the maximum activities observed at pH 10.0 Phanerodontia chrysosporium

pI Value

Organism Comment pI Value Maximum pI Value
Phanerodontia chrysosporium PcALDH1, estimated from sequence
-
 5.9
Phanerodontia chrysosporium PcALDH2, estimated from sequence
-
 6.1

Expression

Organism Comment Expression
Phanerodontia chrysosporium up-regulation of PcALDH1 and PcALDH2 in response to exogenous addition of vanillin, which is an abundant key intermediate in the lignin biodegradation process, basidiomycetes presumably respond to vanillin by activating and optimizing ligninolytic processes up

General Information

General Information Comment Organism
metabolism results suggest that PcALDHs metabolize aryl-aldehyde compounds generated during fungal degradation of lignin and various aromatic xenobiotics Phanerodontia chrysosporium