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Literature summary for 1.2.1.28 extracted from

  • Long, M.C.; Nagegowda, D.A.; Kaminaga, Y.; Ho, K.K.; Kish, C.M.; Schnepp, J.; Sherman, D.; Weiner, H.; Rhodes, D.; Dudareva, N.
    Involvement of snapdragon benzaldehyde dehydrogenase in benzoic acid biosynthesis (2009), Plant J., 59, 256-265.
    View publication on PubMed
Search for more literature for 1.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET-28a+ for expression in Escherichia coli BL21 Rosetta cells, into the pEF1-LIS binary vector for transformation of petunia flowers with Agrobacterium tumefaciens, for subcellular localization experiments in Arabidopsis protoplasts GFP fusion proteins are constructed using the vector p326-SGFP Antirrhinum majus

Inhibitors

Inhibitors Comment Organism Structure
Disulfiram
-
 Antirrhinum majus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00137
-
 benzaldehyde
-
 Antirrhinum majus
0.00201
-
 acetaldehyde
-
 Antirrhinum majus
0.00535
-
 phenylacetaldehyde
-
 Antirrhinum majus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Antirrhinum majus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54500
-
 monomer Antirrhinum majus
232400
-
 tetramer, determined by gel filtration Antirrhinum majus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzaldehyde + NAD+ + H2O Antirrhinum majus
-
 benzoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Antirrhinum majus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography on Ni-NTA agarose Antirrhinum majus

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Antirrhinum majus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate 3-hydroxybenzaldehyde, relative activity 11%, benzaldehyde 100% Antirrhinum majus
additional information
-
 substrate acetaldehyde, relative activity 309%, benzaldehyde 100% Antirrhinum majus
additional information
-
 substrate formaldehyde, relative activity 22%, benzaldehyde 100% Antirrhinum majus
additional information
-
 substrate hexanal, relative activity 50%, benzaldehyde 100% Antirrhinum majus
additional information
-
 substrate m-anisaldehyde, relative activity 25%, benzaldehyde 100% Antirrhinum majus
additional information
-
 substrate p-anisaldehyde, relative activity 31%, benzaldehyde 100% Antirrhinum majus
additional information
-
 substrate phenylacetaldehyde, relative activity 62%, benzaldehyde 100% Antirrhinum majus
additional information
-
 substrate propionaldehyde, relative activity 165%, benzaldehyde 100% Antirrhinum majus
additional information
-
 substrate salicylaldehyde, relative activity 9%, benzaldehyde 100% Antirrhinum majus
additional information
-
 substrate trans-cinnamaldehyde, relative activity 47%, benzaldehyde 100% Antirrhinum majus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzaldehyde + NAD+ + H2O
-
 Antirrhinum majus 3-hydroxybenzoate + NADH + H+
-
 ?
4-methoxybenzaldehyde + NAD+ + H2O
-
 Antirrhinum majus 4-methoxybenzoate + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O
-
 Antirrhinum majus acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Antirrhinum majus benzoate + NADH + H+
-
 ?
formaldehyde + NAD+ + H2O
-
 Antirrhinum majus formate + NADH + H+
-
 ?
hexanal + NAD+ + H2O
-
 Antirrhinum majus hexanoate + NADH + H+
-
 ?
m-anisaldehyde + NAD+ + H2O
-
 Antirrhinum majus m-anisate + NADH + H+
-
 ?
phenylacetaldehyde + NAD+ + H2O
-
 Antirrhinum majus phenylacetate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O
-
 Antirrhinum majus propionate + NADH + H+
-
 ?
salicylaldehyde + NAD+ + H2O
-
 Antirrhinum majus salicylate + NADH + H+
-
 ?
trans-cinnamaldehyde + NAD+ + H2O
-
 Antirrhinum majus trans-cinnamate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 54500 Antirrhinum majus

Synonyms

Synonyms Comment Organism
BALDH
-
 Antirrhinum majus
benzaldehyde dehydrogenase
-
 Antirrhinum majus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
29
-
 activity assay Antirrhinum majus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
 phenylacetaldehyde
-
 Antirrhinum majus
0.31
-
 benzaldehyde
-
 Antirrhinum majus
2.08
-
 acetaldehyde
-
 Antirrhinum majus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Antirrhinum majus

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Antirrhinum majus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Antirrhinum majus