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Literature summary for 1.2.1.25 extracted from

  • Cui, Q.; Zhou, F.; Liu, W.; Tao, Y.
    Avermectin biosynthesis stable functional expression of branched chain alpha-keto acid dehydrogenase complex from Streptomyces avermitilis in Escherichia coli by selectively regulating individual subunit gene expression (2017), Biotechnol. Lett., 39, 1567-1574 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bkdA, the BKDH complex from Streptomyces avermitilis cannot readily be stably expressed in Escherichia coli. This is mostly due to low yields of active, properly folded enzyme in a complex expression system. Codon optimization, recombinant expression of the enzyme complex in Escherichia coli strain BW25113 by selectively regulating individual subunit gene expression Streptomyces avermitilis

Protein Variants

Protein Variants Comment Organism
additional information improvement of the production of short branched-chain acyl-CoAs for avermectin biosynthesis by functional expression of the branched chain alpha-keto acid dehydrogenase complex (BKDH) from Streptomyces avermitilis, systematic optimization by selectively regulating individual subunit expression in Escherichia coli, codon optimization, method, overview. Synergic synthesis pathway of avermectin precursors. Codon optimization could improve the expression of BkdH and LpdA1 from pRB1a, we further coexpressed the bkdF and bkdG with wild-type or codon optimized bkdH and lpdA1 by constructing vectors pRB01 (wild-type) and pRB02 (optimized). The bkdF-bkdG and bkdH-lpdA1 gene clusters were placed under the control of two independent araBAD promoters in pRB1a. Genes bkdF and bkdG are respectively inserted close to the araBAD promoters so as to enhance their expression Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-methyl-2-oxobutanoate + CoA + NAD+ Streptomyces avermitilis
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2-methylpropanoyl-CoA + CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis Q53592 BKDH complex component E1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-oxobutanoate + CoA + NAD+
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Streptomyces avermitilis 2-methylpropanoyl-CoA + CO2 + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
bkdA
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Streptomyces avermitilis
BKDH
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Streptomyces avermitilis
branched chain alpha-keto acid dehydrogenase complex
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Streptomyces avermitilis

Cofactor

Cofactor Comment Organism Structure
CoA
-
Streptomyces avermitilis
NAD+
-
Streptomyces avermitilis
thiamine diphosphate
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Streptomyces avermitilis

General Information

General Information Comment Organism
physiological function the BKDH complex is a multienzyme complex composed of three catalytic components: a thiamine diphosphate-dependent decarboxylase/dehydrogenase (E1), a lipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3) Streptomyces avermitilis