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Literature summary for 1.2.1.24 extracted from
- Unraveling the function of paralogs of the aldehyde dehydrogenase super family from Sulfolobus solfataricus (2013), Extremophiles, 17, 205-216.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutaric semialdehyde | substrate inhibition | Saccharolobus solfataricus |  |
| NAD+ | substrate inhibition | Saccharolobus solfataricus | |
| succinate semialdehyde | substrate inhibition | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
succinate semialdehyde | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
| 0.3 | - |
2,5-dioxopentanoate | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
| 9.1 | - |
NAD+ | pH 6.5, 70°C | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
3 * 50000, SDS-PAGE | Saccharolobus solfataricus |
| 51500 | - |
3 * 51500, calculated from sequence | Saccharolobus solfataricus |
| 157000 | - |
gel filtration | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97XS9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-dioxopentanoate + NAD+ + H2O | no activity in presence of NADP+ | Saccharolobus solfataricus | ? | - |
? | |
| additional information | formaldehyde, betaine aldehyde and benzaldehyde can not serve as substrates | Saccharolobus solfataricus | ? | - |
? | |
| succinate semialdehyde + NAD+ + H2O | no activity in presence of NADP+ | Saccharolobus solfataricus | succinate + NADH + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 50000, SDS-PAGE | Saccharolobus solfataricus |
| homotrimer | 3 * 51500, calculated from sequence | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SSADH-I | - |
Saccharolobus solfataricus |
| SSO1629 | ordered locus name | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.1 | - |
2,5-dioxopentanoate | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
| 22.6 | - |
succinate semialdehyde | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
| 69 | - |
NAD+ | pH 6.5, 70°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | no activity in presence of NADP+ | Saccharolobus solfataricus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.6 | - |
NAD+ | pH 6.5, 70°C | Saccharolobus solfataricus | |
| 21.4 | - |
2,5-dioxopentanoate | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
| 842 | - |
succinate semialdehyde | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
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Release 2023.1 (January 2023)
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