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Literature summary for 1.2.1.24 extracted from

  • Rothacker, B.; Werr, M.; Ilg, T.
    Molecular cloning, partial genomic structure and functional characterization of succinic semialdehyde dehydrogenase genes from the parasitic insects Lucilia cuprina and Ctenocephalides felis (2008), Insect Mol. Biol., 17, 279-291.
    View publication on PubMed
Search for more literature for 1.2.1.24

Application

Application Comment Organism
additional information genomic copy of the SSADH gene contains two introns. Multiple SSADH gene copies are present in the genome Lucilia cuprina
additional information genomic copy of the SSADH gene is devoid of introns. Multiple SSADH gene copies are present in the genome Ctenocephalides felis

Cloned(Commentary)

Cloned (Comment) Organism
into vectors pQE30 and into pMalc2x. Expression in Escherichia coli strains M15 and TB1 Ctenocephalides felis
into vectors pQE30 and into pMalc2x. Expression in Escherichia coli strains M15 and TB1 Lucilia cuprina

Inhibitors

Inhibitors Comment Organism Structure
succinate semialdehyde inhibition by the succinate semialdehyde substrate is starting already at a concentration as low as 0.02 mM Ctenocephalides felis
succinate semialdehyde inhibition by the succinate semialdehyde substrate is starting already at a concentration as low as 0.02 mM Lucilia cuprina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 succinate semialdehyde
-
 Ctenocephalides felis
0.0027
-
 succinate semialdehyde
-
 Lucilia cuprina
0.0121
-
 4-carboxybenzaldehyde
-
 Ctenocephalides felis
0.017
-
 3-carboxybenzaldehyde
-
 Ctenocephalides felis
0.025
-
 pentanal
-
 Ctenocephalides felis
0.076
-
 NAD+
-
 Ctenocephalides felis
0.0776
-
 4-carboxybenzaldehyde
-
 Lucilia cuprina
0.0827
-
 pentanal
-
 Lucilia cuprina
0.105
-
 Butanal
-
 Ctenocephalides felis
0.112
-
 3-carboxybenzaldehyde
-
 Lucilia cuprina
0.241
-
 benzaldehyde
-
 Ctenocephalides felis
0.348
-
 NAD+
-
 Lucilia cuprina
0.552
-
 NADP+
-
 Lucilia cuprina
1.606
-
 propanal
-
 Ctenocephalides felis
4.076
-
 acetaldehyde
-
 Ctenocephalides felis
4.4
-
 NADP+
-
 Ctenocephalides felis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 deduced protein without the mitochondria import sequence Lucilia cuprina
52100
-
 deduced protein without the mitochondria import sequence Ctenocephalides felis
55400
-
 deduced protein with the mitochondria import sequence Ctenocephalides felis
55400
-
 deduced protein with the mitochondria import sequence Lucilia cuprina

Organism

Organism UniProt Comment Textmining
Ctenocephalides felis
-
-
-
Lucilia cuprina B0JFD4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-carboxybenzaldehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis 3-carboxybenzoate + NAD(P)H + H+
-
 ?
3-carboxybenzaldehyde + NAD(P)+ + H2O
-
 Lucilia cuprina 3-carboxybenzoate + NAD(P)H + H+
-
 ?
4-carboxybenzaldehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis 4-carboxybenzoate + NAD(P)H + H+
-
 ?
4-carboxybenzaldehyde + NAD(P)+ + H2O
-
 Lucilia cuprina 4-carboxybenzoate + NAD(P)H + H+
-
 ?
acetaldehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis acetate + NAD(P)H + H+
-
 ?
benzaldehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis benzoate + NAD(P)H + H+
-
 ?
butanal + NAD(P)+ + H2O
-
 Ctenocephalides felis butanoate + NAD(P)H + H+
-
 ?
pentanal + NAD(P)+ + H2O
-
 Ctenocephalides felis pentanoate + NAD(P)H + H+
-
 ?
pentanal + NAD(P)+ + H2O
-
 Lucilia cuprina pentanoate + NAD(P)H + H+
-
 ?
propanal + NAD(P)+ + H2O
-
 Ctenocephalides felis propanoate + NAD(P)H + H+
-
 ?
succinate semialdehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis succinate + NAD(P)H + H+
-
 ?
succinate semialdehyde + NAD(P)+ + H2O
-
 Lucilia cuprina succinate + NAD(P)H + H+
-
 ?
succinate semialdehyde + NAD+ + H2O
-
 Ctenocephalides felis succinate + NADH + H+
-
 r
succinate semialdehyde + NAD+ + H2O
-
 Lucilia cuprina succinate + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
SSADH
-
 Ctenocephalides felis
SSADH
-
 Lucilia cuprina
succinic semialdehyde dehydrogenase
-
 Ctenocephalides felis
succinic semialdehyde dehydrogenase
-
 Lucilia cuprina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Lucilia cuprina

pH Range

pH Minimum pH Maximum Comment Organism
8 9
-
 Ctenocephalides felis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Ctenocephalides felis
NAD+
-
 Lucilia cuprina
NADP+
-
 Ctenocephalides felis
NADP+
-
 Lucilia cuprina