Literature summary for 1.2.1.24 extracted from

Kang, J.H.; Park, Y.B.; Huh, T.L.; Lee, W.H.; Choi, M.S.; Kwon, O.S.
High-level expression and characterization of the recombinant enzyme, and tissue distribution of human succinic semialdehyde dehydrogenase (2005), Protein Expr. Purif., 44, 16-22.

Search for more literature for 1.2.1.24

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli as His-tag fusion protein	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
NADH	NADH is a strong competitive inhibitor with respect to NAD+ and behaves as a non-competitive inhibitor with respect to succinate semialdehyde	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.3	-	succinate semialdehyde	50 microM succinate semialdehyde, 3 mM NAD+, 0.1 M sodium diphosphate pH 8.5, 25°C	Homo sapiens
125	-	NAD+	50 microM succinate semialdehyde, 3 mM NAD+, 0.1 M sodium diphosphate pH 8.5, 25°C	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52000	-	4 * 52000, gel-filtration	Homo sapiens
52230	-	calculated from sequence, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	low mRNA expression compared to other tissues	Homo sapiens	-
kidney	mRNA expression	Homo sapiens	-
liver	highest mRNA expression	Homo sapiens	-
skeletal muscle	mRNA expression	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinate semialdehyde + NAD+ + H2O	-	Homo sapiens	succinate + NADH	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 52000, gel-filtration	Homo sapiens

Synonyms

Synonyms	Comment	Organism
SSADH	-	Homo sapiens
succinic semialdehyde dehydrogenase	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
8	9	at least 80% of its optimal activity	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.1	-	NADH	succinate semialdehyde as varied substrate	Homo sapiens
0.128	-	NADH	NAD+ as varied substrate	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	computer prediction	-	7.66

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Release 2023.1 (January 2023)