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Literature summary for 1.2.1.23 extracted from

  • Dunkerton, J.; James, S.P.
    Purification of 2-oxoaldehyde dehydrogenase and its dependence on unusual amines (1975), Biochem. J., 149, 609-617.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.23

Activating Compound

Activating Compound Comment Organism Structure
2-amino-2-methylpropan-1,3-diol activation of aldehyde oxidation Ovis aries
2-amino-2-methylpropan-1-ol activation of aldehyde oxidation Ovis aries
2-Aminobutan-1-ol activation of aldehyde oxidation Ovis aries
2-aminopropane activation of aldehyde oxidation Ovis aries
2-aminopropane-1,3-diol activation of aldehyde oxidation Ovis aries
L-2-Aminopropan-1-ol activation of aldehyde oxidation Ovis aries
phosphate activation Ovis aries
Tris activation of aldehyde oxidation Ovis aries

Inhibitors

Inhibitors Comment Organism Structure
3',5'-cyclic AMP weak inhibition Ovis aries
ADP weak inhibition Ovis aries
AMP weak inhibition Ovis aries
barbiturates at large concentrations, 6-46 mM Ovis aries
diethanolamine causes 40% inhibition of the enzyme activated by L-2-aminopropan-1-ol at a concentration of 50 mM Ovis aries
Nitro-alcohols analogues of the activating amines, at large concentrations, 66-83 mM Ovis aries
phosphate inhibits the enzyme prepared from the acetone-dried liver extract, 10-20 mM Ovis aries
Tris(hydroxymethyl)nitromethane 50% inhibition at a concentration of 139 mM Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 NAD+ assay mixture containing 40 mM L-2-aminopropan-1-ol, 5 mM methylglyoxal and 10 mM potassium phosphate Ovis aries
0.32
-
 NAD+ assay mixture containing 40 mM L-2-aminopropan-1-ol, 5 mM methylglyoxal, no potassium phosphate Ovis aries
2.08
-
 methylglyoxal assay mixture containing 40 mM L-2-aminopropan-1-ol and 10 mM potassium phosphate Ovis aries
2.08
-
 methylglyoxal amine as activator in aldehyde oxidation, same value for methylglyoxal as substrate, assay mixture containing 10 mM potassium phosphate Ovis aries
2.08
-
 methylglyoxal in the presence of L-2-aminopropan-1-ol (Km 5.0 mM) and 10 mM phosphate, at pH 9.3 and 25°C Ovis aries
2.6
-
 methylglyoxal amine as activator in aldehyde oxidation Ovis aries
2.6
-
 methylglyoxal assay mixture containing 40 mM 2-amino-2-methylpropan-1-ol and 10 mM potassium phosphate Ovis aries
2.6
-
 methylglyoxal in the presence of 2-amino-2-methylpropan-1-ol (Km 15 mM), at pH 9.3 and 25°C Ovis aries
3.8
-
 methylglyoxal amine as activator in aldehyde oxidation Ovis aries
3.8
-
 methylglyoxal assay mixture containing 40 mM 2-aminobutan-1-ol and 10 mM potassium phosphate Ovis aries
3.8
-
 methylglyoxal in the presence of 2-aminobutan-1-ol (Km 20 mM), at pH 9.3 and 25°C Ovis aries
4
-
 methylglyoxal amine as activator in aldehyde oxidation Ovis aries
4
-
 methylglyoxal assay mixture containing 40 mM Tris and 10 mM potassium phosphate Ovis aries
4
-
 methylglyoxal in the presence of Tris (Km 2.4 mM), at pH 9.3 and 25°C Ovis aries
5
-
 methylglyoxal amine as activator in aldehyde oxidation Ovis aries
5
-
 methylglyoxal in the presence of 2-amino-2-methylpropan-1,3-diol (Km 4.3 mM), at pH 9.3 and 25°C Ovis aries
5.5
-
 methylglyoxal amine as activator in aldehyde oxidation Ovis aries
5.5
-
 methylglyoxal assay mixture containing 40 mM 2-aminopropane-1,3-diol and 10 mM potassium phosphate Ovis aries
5.5
-
 methylglyoxal in the presence of 2-aminopropane-1,3-diol (Km 7.7 mM), at pH 9.3 and 25°C Ovis aries
7.14
-
 methylglyoxal assay mixture containing 40 mM L-2-aminopropan-1-ol, no potassium phosphate Ovis aries
7.14
-
 methylglyoxal amine as activator in aldehyde oxidation, assay mixture containing 5 mM methylglyoxal, no potassium phosphate Ovis aries
7.14
-
 methylglyoxal in the presence of L-2-aminopropan-1-ol (Km 36 mM), without phosphate, at pH 9.3 and 25°C Ovis aries
16.6
-
 2-aminopropane amine as activator in aldehyde oxidation Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + NAD+ + H2O Ovis aries i.e. pyruvaldehyde pyruvate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, Phosphocellulose, Sephadex G-200, Hydroxylapatite, and a second DEAE-cellulose step Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1334
-
-
 Ovis aries

Storage Stability

Storage Stability Organism
4°C, pH 7.4, 50 mM NaCl, 5 mM 2-amino-2-methylpropan-1,3-diol, 20 days Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoaldehydes + NAD+ + H2O enzyme from sheep or rat liver also active with NADP+, see also EC 1.2.1.49 Ovis aries 2-oxo acids + NADH
-
 ?
methylglyoxal + NAD+ + H2O i.e. pyruvaldehyde Ovis aries pyruvate + NADH + H+
-
 ?
additional information the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme Ovis aries ?
-
 ?
additional information the presence of amines like Tris, 2-amino-2-methylpropan-1,3-diol, 2-amino-2-methylpropan-1-ol, 2-aminopropane-1,3-diol, 2-aminopropane or 2-aminobutan-1-ol is necessary to activate the enzyme Ovis aries ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
 Ovis aries

pH Range

pH Minimum pH Maximum Comment Organism
7.8
-
 not active below Ovis aries

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Ovis aries
NADP+ enzyme from sheep or rat liver, see also EC 1.2.1.49 Ovis aries