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Literature summary for 1.2.1.22 extracted from

  • Watanabe, S.; Piyanart, S.; Makino, K.
    Metabolic fate of L-lactaldehyde derived from an alternative L-rhamnose pathway (2008), FEBS J., 275, 5139-5149.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli cells as a His6-tagged enzyme Azotobacter vinelandii
overexpression in Escherichia coli cells as a His6-tagged enzyme Scheffersomyces stipitis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Scheffersomyces stipitis 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Scheffersomyces stipitis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
SDS-PAGE Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactaldehyde + NAD(P)+ + H2O Scheffersomyces stipitis
-
(S)-lactate + NAD(P)H + 2 H+
-
?
(S)-lactaldehyde + NAD+ + H2O Azotobacter vinelandii
-
(S)-lactate + NADH + 2 H+
-
?
(S)-lactaldehyde + NAD+ + H2O Azotobacter vinelandii NBRC 102612
-
(S)-lactate + NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Azotobacter vinelandii NBRC 102612
-
-
-
Scheffersomyces stipitis
-
-
-
Scheffersomyces stipitis A3LNE3
-
-
Scheffersomyces stipitis A3M013
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-chelating affinity column Azotobacter vinelandii
nickel-chelating affinity column Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.355
-
purified recombinant enzyme, S-lactaldehyde, NADP+ Scheffersomyces stipitis
1.65
-
purified recombinant enzyme, (S)-lactaldehyde, NADP+ Scheffersomyces stipitis
2.1
-
purified recombinant enzyme, R-lactaldehyde, NAD+ Scheffersomyces stipitis
2.28
-
purified recombinant enzyme, (R)-D-lactaldehyde, NAD+ Azotobacter vinelandii
3.64
-
purified recombinant enzyme, S-lactaldehyde, NAD+ Scheffersomyces stipitis
4.28
-
purified recombinant enzyme, glycolaldehyde, NAD+ Scheffersomyces stipitis
4.43
-
purified recombinant enzyme, (R)-lactaldehyde, NAD+ Scheffersomyces stipitis
6.95
-
purified recombinant enzyme, (S)-lactaldehyde, NAD+ Scheffersomyces stipitis
8.94
-
purified recombinant enzyme, glycolaldehyde, NAD+ Scheffersomyces stipitis
11.5
-
purified recombinant enzyme, glycolaldehyde, NAD+ Azotobacter vinelandii
17.2
-
purified recombinant enzyme, (S)-lactaldehyde, NAD+ Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactaldehyde + NAD+ + H2O 54% of the activity of L-lactaldehyde Scheffersomyces stipitis (R)-lactate + NADH + 2 H+
-
?
(R)-lactaldehyde + NAD+ + H2O 75% of the activity of (S)-lactaldehyde Scheffersomyces stipitis (R)-lactate + NADH + 2 H+
-
?
(R)-lactaldehyde + NAD+ + H2O 8.6% of the activity of (S)-lactaldehyde Azotobacter vinelandii (R)-lactate + NADH + 2 H+
-
?
(R)-lactaldehyde + NAD+ + H2O 8.6% of the activity of (S)-lactaldehyde Azotobacter vinelandii NBRC 102612 (R)-lactate + NADH + 2 H+
-
?
(S)-lactaldehyde + NAD(P)+ + H2O
-
Scheffersomyces stipitis (S)-lactate + NAD(P)H + 2 H+
-
?
(S)-lactaldehyde + NAD+ + H2O best substrate Azotobacter vinelandii (S)-lactate + NADH + H+
-
?
(S)-lactaldehyde + NAD+ + H2O best substrate Scheffersomyces stipitis (S)-lactate + NADH + H+
-
?
(S)-lactaldehyde + NAD+ + H2O best substrate Azotobacter vinelandii NBRC 102612 (S)-lactate + NADH + H+
-
?
(S)-lactaldehyde + NAD+ + H2O
-
Azotobacter vinelandii (S)-lactate + NADH + 2 H+
-
?
(S)-lactaldehyde + NAD+ + H2O
-
Azotobacter vinelandii NBRC 102612 (S)-lactate + NADH + 2 H+
-
?
glycolaldehyde + NAD+ + H2O
-
Azotobacter vinelandii glycolate + NADH + 2 H+
-
?
glycolaldehyde + NAD+ + H2O
-
Scheffersomyces stipitis glycolate + NADH + 2 H+
-
?
glycolaldehyde + NAD+ + H2O
-
Azotobacter vinelandii NBRC 102612 glycolate + NADH + 2 H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Scheffersomyces stipitis

Synonyms

Synonyms Comment Organism
AvLADH
-
Azotobacter vinelandii
L-lactaldehyde dehydrogenase
-
Azotobacter vinelandii
L-lactaldehyde dehydrogenase
-
Scheffersomyces stipitis
LADH
-
Azotobacter vinelandii
LADH
-
Scheffersomyces stipitis
PsLADH
-
Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ Azotobacter vinelandii
NAD+
-
Azotobacter vinelandii
NAD+
-
Scheffersomyces stipitis
NADP+
-
Scheffersomyces stipitis