Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli cells as a His6-tagged enzyme | Azotobacter vinelandii |
overexpression in Escherichia coli cells as a His6-tagged enzyme | Scheffersomyces stipitis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Scheffersomyces stipitis | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Scheffersomyces stipitis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Scheffersomyces stipitis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactaldehyde + NAD(P)+ + H2O | Scheffersomyces stipitis | - |
(S)-lactate + NAD(P)H + 2 H+ | - |
? | |
(S)-lactaldehyde + NAD+ + H2O | Azotobacter vinelandii | - |
(S)-lactate + NADH + 2 H+ | - |
? | |
(S)-lactaldehyde + NAD+ + H2O | Azotobacter vinelandii NBRC 102612 | - |
(S)-lactate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Azotobacter vinelandii NBRC 102612 | - |
- |
- |
Scheffersomyces stipitis | - |
- |
- |
Scheffersomyces stipitis | A3LNE3 | - |
- |
Scheffersomyces stipitis | A3M013 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelating affinity column | Azotobacter vinelandii |
nickel-chelating affinity column | Scheffersomyces stipitis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.355 | - |
purified recombinant enzyme, S-lactaldehyde, NADP+ | Scheffersomyces stipitis |
1.65 | - |
purified recombinant enzyme, (S)-lactaldehyde, NADP+ | Scheffersomyces stipitis |
2.1 | - |
purified recombinant enzyme, R-lactaldehyde, NAD+ | Scheffersomyces stipitis |
2.28 | - |
purified recombinant enzyme, (R)-D-lactaldehyde, NAD+ | Azotobacter vinelandii |
3.64 | - |
purified recombinant enzyme, S-lactaldehyde, NAD+ | Scheffersomyces stipitis |
4.28 | - |
purified recombinant enzyme, glycolaldehyde, NAD+ | Scheffersomyces stipitis |
4.43 | - |
purified recombinant enzyme, (R)-lactaldehyde, NAD+ | Scheffersomyces stipitis |
6.95 | - |
purified recombinant enzyme, (S)-lactaldehyde, NAD+ | Scheffersomyces stipitis |
8.94 | - |
purified recombinant enzyme, glycolaldehyde, NAD+ | Scheffersomyces stipitis |
11.5 | - |
purified recombinant enzyme, glycolaldehyde, NAD+ | Azotobacter vinelandii |
17.2 | - |
purified recombinant enzyme, (S)-lactaldehyde, NAD+ | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactaldehyde + NAD+ + H2O | 54% of the activity of L-lactaldehyde | Scheffersomyces stipitis | (R)-lactate + NADH + 2 H+ | - |
? | |
(R)-lactaldehyde + NAD+ + H2O | 75% of the activity of (S)-lactaldehyde | Scheffersomyces stipitis | (R)-lactate + NADH + 2 H+ | - |
? | |
(R)-lactaldehyde + NAD+ + H2O | 8.6% of the activity of (S)-lactaldehyde | Azotobacter vinelandii | (R)-lactate + NADH + 2 H+ | - |
? | |
(R)-lactaldehyde + NAD+ + H2O | 8.6% of the activity of (S)-lactaldehyde | Azotobacter vinelandii NBRC 102612 | (R)-lactate + NADH + 2 H+ | - |
? | |
(S)-lactaldehyde + NAD(P)+ + H2O | - |
Scheffersomyces stipitis | (S)-lactate + NAD(P)H + 2 H+ | - |
? | |
(S)-lactaldehyde + NAD+ + H2O | best substrate | Azotobacter vinelandii | (S)-lactate + NADH + H+ | - |
? | |
(S)-lactaldehyde + NAD+ + H2O | best substrate | Scheffersomyces stipitis | (S)-lactate + NADH + H+ | - |
? | |
(S)-lactaldehyde + NAD+ + H2O | best substrate | Azotobacter vinelandii NBRC 102612 | (S)-lactate + NADH + H+ | - |
? | |
(S)-lactaldehyde + NAD+ + H2O | - |
Azotobacter vinelandii | (S)-lactate + NADH + 2 H+ | - |
? | |
(S)-lactaldehyde + NAD+ + H2O | - |
Azotobacter vinelandii NBRC 102612 | (S)-lactate + NADH + 2 H+ | - |
? | |
glycolaldehyde + NAD+ + H2O | - |
Azotobacter vinelandii | glycolate + NADH + 2 H+ | - |
? | |
glycolaldehyde + NAD+ + H2O | - |
Scheffersomyces stipitis | glycolate + NADH + 2 H+ | - |
? | |
glycolaldehyde + NAD+ + H2O | - |
Azotobacter vinelandii NBRC 102612 | glycolate + NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Scheffersomyces stipitis |
Synonyms | Comment | Organism |
---|---|---|
AvLADH | - |
Azotobacter vinelandii |
L-lactaldehyde dehydrogenase | - |
Azotobacter vinelandii |
L-lactaldehyde dehydrogenase | - |
Scheffersomyces stipitis |
LADH | - |
Azotobacter vinelandii |
LADH | - |
Scheffersomyces stipitis |
PsLADH | - |
Scheffersomyces stipitis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Azotobacter vinelandii | |
NAD+ | - |
Azotobacter vinelandii | |
NAD+ | - |
Scheffersomyces stipitis | |
NADP+ | - |
Scheffersomyces stipitis |