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Literature summary for 1.2.1.21 extracted from

  • Morita, H.; Yamamoto, K.; Tani, Y.; Ogata, K.; Yamada, H.
    Glycoladehyde dehydrogenase, an enzyme related to vitamin B6 biosynthesis, from Bacillus subtilis (1979), Agric. Biol. Chem., 43, 317-323.
No PubMed abstract available
Search for more literature for 1.2.1.21

General Stability

General Stability Organism
glycerol stabilizes Bacillus subtilis
unstable enzyme, not stabilized by PMSF Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 1 mM, 63% inhibition Bacillus subtilis
HgCl2
-
 Bacillus subtilis
p-chloromercuribenzoate 0.1 mM, 36% inhibition Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00023
-
 benzaldehyde
-
 Bacillus subtilis
0.023
-
 propionaldehyde
-
 Bacillus subtilis
0.038
-
 acetaldehyde
-
 Bacillus subtilis
0.25
-
 glycolaldehyde
-
 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis IFO 3007
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isozymes A and B Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.322
-
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
-20°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
 Bacillus subtilis acetate + NADH + H+
-
 r
acetaldehyde + NAD+ + H2O
-
 Bacillus subtilis IFO 3007 acetate + NADH + H+
-
 r
benzaldehyde + NAD+ + H2O good substrate for oxidizing reaction, bad substrate for reducing reaction, very low activity with NADP+ Bacillus subtilis benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O good substrate for oxidizing reaction, bad substrate for reducing reaction, very low activity with NADP+ Bacillus subtilis IFO 3007 benzoate + NADH + H+
-
 ?
DL-glyceraldehyde + NAD+ + H2O
-
 Bacillus subtilis 2,3-dihydroxypropanoic acid + NADH + H+
-
 ?
glycolaldehyde + NAD+ + H2O
-
 Bacillus subtilis glycolate + NADH
-
 r
glycolaldehyde + NAD+ + H2O
-
 Bacillus subtilis IFO 3007 glycolate + NADH
-
 r
glycolaldehyde + NADP+ + H2O 87.4% of activity with NAD+, 595% of activity with NADH in reverse reaction Bacillus subtilis glycolate + NADPH + H+
-
 r
glycolaldehyde + NADP+ + H2O 87.4% of activity with NAD+, 595% of activity with NADH in reverse reaction Bacillus subtilis IFO 3007 glycolate + NADPH + H+
-
 r
hydroxypyruvate + NAD+ + H2O
-
 Bacillus subtilis ?
-
 ?
propionaldehyde + NAD+ + H2O
-
 Bacillus subtilis propionate + NADH
-
 ?
propionaldehyde + NAD+ + H2O
-
 Bacillus subtilis IFO 3007 propionate + NADH
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus subtilis
NADP+ 87.4% of activity with NAD+ in glycolaldehyde oxidation, NADPH is 6 times more effectiv than NADH in glycolate reduction Bacillus subtilis