General Stability | Organism |
---|---|
2-mercaptoethanol and NAD+, no stabilization | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Carboxy-2'-hydroxy-5'-sulfoformazylbenzene | zincon | Pseudomonas putida | |
iodoacetamide | - |
Pseudomonas putida | |
monoiodoacetate | slowly | Pseudomonas putida | |
N-ethylmaleimide | - |
Pseudomonas putida | |
p-chloromercuribenzoate | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
Valeraldehyde | - |
Pseudomonas putida | |
0.016 | - |
Butyraldehyde | - |
Pseudomonas putida | |
0.13 | - |
4-guanidinobutyraldehyde | pH 9.5 | Pseudomonas putida | |
0.16 | - |
4-guanidinobutyraldehyde | pH 8.0 | Pseudomonas putida | |
0.2 | - |
5-Aminovaleraldehyde | - |
Pseudomonas putida | |
0.26 | - |
4-Aminobutyraldehyde | pH 8.0 | Pseudomonas putida | |
0.83 | - |
4-Aminobutyraldehyde | pH 9.5 | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
4 * 57000, SDS-PAGE | Pseudomonas putida |
83000 | - |
gel filtration | Pseudomonas putida |
240000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanal + NAD+ + H2O | Pseudomonas putida | involved in the arginine decarboxylase pathway | 4-aminobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
A.T.C.C. 12633 | - |
Purification (Comment) | Organism |
---|---|
using column chromatography on DEAE-cellulose, DEAE-Toyopearl, Toyopearl HW-55 and Affi-Gel Blue | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminopropanal + NAD+ + H2O | - |
Pseudomonas putida | 3-aminopropanoate + NADH + H+ | - |
? | |
4-aminobutanal + NAD+ + H2O | - |
Pseudomonas putida | 4-aminobutanoate + NADH + H+ | - |
? | |
4-aminobutanal + NAD+ + H2O | involved in the arginine decarboxylase pathway | Pseudomonas putida | 4-aminobutanoate + NADH + H+ | - |
? | |
4-guanidinobutyraldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-guanidinobutyrate + NADH + H+ | - |
? | |
4-ureidobutyraldehyde + NAD+ | - |
Pseudomonas putida | 4-ureidobutyrate + NADH + H+ | - |
? | |
5-aminovaleraldehyde + NAD+ + H2O | - |
Pseudomonas putida | 5-aminovalerate + NADH + H+ | - |
? | |
butyraldehyde + NAD+ + H2O | - |
Pseudomonas putida | butyrate + NADH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | - |
Pseudomonas putida | propionate + NADH + H+ | - |
? | |
valeraldehyde + NAD+ + H2O | - |
Pseudomonas putida | valerate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 57000, SDS-PAGE | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min, pH 5.0-8.0, stable | Pseudomonas putida |
30 | - |
for 30 min, in 100 mM Na-acetate buffer, pH 5, allmost all activity retained | Pseudomonas putida |
30 | - |
for 30 min, in 100 mM K-phosphate buffer, pH 7, allmost all activity retained | Pseudomonas putida |
30 | - |
for 30 min, in 100 mM Na-bicine buffer, pH 8, allmost all activity retained | Pseudomonas putida |
60 | - |
pH 7.0, 15 min, 35% loss of activity | Pseudomonas putida |
60 | - |
for 15 min, in 100 mM K-phosphate buffer, pH 7, 35% of activity lost | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
optimum toward 4-aminobutyraldehyde | Pseudomonas putida |
8.5 | 9.5 | optimum toward 4-guanidinobutyraldehyde and 5-aminovaleraldehyde | Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | 4-aminobutyraldehyde, at pH 6.5: about 30% of activity maximum, at pH 9.0: about 70% of activity maximum | Pseudomonas putida |
7 | 10 | 4-guanidinobutyraldehyde, at pH 7.0: about 35% of activity maximum, at pH 10.0: about 50% of activity maximum | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
most stable at neutral pH | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | highly specific for | Pseudomonas putida | |
NADP+ | rate of dehydrogenation about 4% those with NAD+ | Pseudomonas putida |