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Literature summary for 1.2.1.17 extracted from

  • Quayle, J.R.; Taylor, G.A.
    Carbon assimilation by Pseudomonas oxalaticus (OX1) (1961), Biochem. J., 78, 611-615.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.17

Inhibitors

Inhibitors Comment Organism Structure
Tris buffer
-
 Cupriavidus oxalaticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glyoxylate + CoA + NADP+ Pseudomonas sp.
-
 oxalyl-CoA + NADPH
-
 r
glyoxylate + CoA + NADP+ Cupriavidus oxalaticus
-
 oxalyl-CoA + NADPH
-
 r

Organism

Organism UniProt Comment Textmining
Cupriavidus oxalaticus
-
-
-
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using protamine sulfate treatment, ammonium sulfate precipitation and dialysis, alumina C-gamma adsorption, concentration of the enzyme by ammonium sulfate precipitation followed by dialysis and ion exchange chromatography on DEAE-cellulose Cupriavidus oxalaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2
-
-
 Cupriavidus oxalaticus
7.2
-
-
 Cupriavidus oxalaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + CoA + NADP+
-
 Pseudomonas sp. oxalyl-CoA + NADPH
-
 r
glyoxylate + CoA + NADP+
-
 Cupriavidus oxalaticus oxalyl-CoA + NADPH
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 glyoxylate + CoA + NADP+ Cupriavidus oxalaticus

Cofactor

Cofactor Comment Organism Structure
CoA
-
 Pseudomonas sp.
CoA specific for Cupriavidus oxalaticus
NADP+
-
 Pseudomonas sp.
NADP+ specific for Cupriavidus oxalaticus