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Literature summary for 1.2.1.16 extracted from

  • Zheng, H.; Beliavsky, A.; Tchigvintsev, A.; Brunzelle, J.; Brown, G.; Flick, R.; Evdokimova, E.; Wawrzak, Z.; Mahadevan, R.; Anderson, W.; Savchenko, A.; Yakunin, A.
    Structure and activity of the NAD(P)+-dependent succinate semialdehyde dehydrogenase YneI from Salmonella typhimurium (2013), Proteins Struct. Funct. Bioinform., 81, 1031-1041.
    View publication on PubMed
Search for more literature for 1.2.1.16

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of apoenzyme and in complex with NAD+, to 1.85 and 1.9 A resolution. Two domain protein with the active site located in the interdomain interface. The NAD+ molecule is bound in the long channel with its nicotinamide ring positioned close to the side chain of the catalytic Cys268 Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
D426A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
E442A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
F138A decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
K160A Lys160 contributes to the enzyme preference to NAD+, strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
K160E Lys160 contributes to the enzyme preference to NAD+, strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
K92A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
N193A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
R145A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
R428A activity similar to wild-type Salmonella enterica subsp. enterica serovar Typhimurium
S425A decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
W141A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
Succinic semialdehyde substrate inhibition above 0.1 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 succinate semialdehyde mutant R428A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.027
-
 succinate semialdehyde wild-type, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.043
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.047
-
 succinate semialdehyde wild-type, cofactor NADP+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.12
-
 succinate semialdehyde mutant F138A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.14
-
 succinate semialdehyde mutant E442A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.15
-
 NAD+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.22
-
 succinate semialdehyde mutant S425A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.23
-
 succinate semialdehyde mutant D426A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.48
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.77
-
 succinate semialdehyde mutant W141A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.4
-
 NADP+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.49
-
 succinate semialdehyde mutant R145A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.6
-
 succinate semialdehyde mutant K92A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
10
-
 NAD+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
11
-
 NAD+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
26
-
 NADP+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
27
-
 NADP+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium Q8ZPI3
-
-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 Q8ZPI3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 succinate + NADPH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
YneI
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 succinate semialdehyde mutant D426A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2 8 succinate semialdehyde mutant R428A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.1
-
 NADP+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.6
-
 NADP+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
14
-
 NAD+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
21
-
 NADP+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
26
-
 succinate semialdehyde mutant F138A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
38
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
39
-
 NAD+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
45
-
 succinate semialdehyde mutant R145A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
46
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
55
-
 succinate semialdehyde wild-type, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
66
-
 succinate semialdehyde mutant W141A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
68
-
 succinate semialdehyde mutant S425A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
73
-
 succinate semialdehyde mutant K92A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
80
-
 NAD+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
83
-
 succinate semialdehyde wild-type, cofactor NADP+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
164
-
 succinate semialdehyde mutant E442A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
NAD+ affinity to NAD+ is 10 times higher than to NADP+ Salmonella enterica subsp. enterica serovar Typhimurium
NADP+ affinity to NAD+ is 10 times higher than to NADP+ Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 NADP+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.77
-
 NADP+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.3
-
 succinate semialdehyde mutant D426A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
3.8
-
 NAD+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
7.3
-
 NAD+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
8.6
-
 NADP+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
18
-
 succinate semialdehyde mutant R145A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
28
-
 succinate semialdehyde mutant K92A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
86
-
 succinate semialdehyde mutant W141A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
95
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
97
-
 NAD+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
210
-
 succinate semialdehyde mutant F138A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
310
-
 succinate semialdehyde mutant S425A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
880
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1100
-
 succinate semialdehyde mutant E442A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1300
-
 succinate semialdehyde mutant R428A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1800
-
 succinate semialdehyde wild-type, cofactor NADP+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2000
-
 succinate semialdehyde wild-type, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium