Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-aminobutyrate | induction during growth with 4-aminobutyrate (GABA) | Cupriavidus necator |
General Stability | Organism |
---|---|
enzyme is labile (50% loss of activity overnight) but can be largely stabilized (in crude extract only) by the addition of 10 mM dithiothreitol 1.2.1.16 | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
3-sulfopropanal | Km apparent value | Cupriavidus necator | |
0.23 | - |
NAD+ | Km apparent value | Cupriavidus necator | |
0.35 | - |
NADP+ | Km apparent value | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
SDS-PAGE | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-sulfopropanal + NAD(P)+ + H2O | Cupriavidus necator | - |
3-sulfopropanoate + NAD(P)H + 2 H+ | - |
? | |
succinate semialdehyde + NAD(P)+ + H2O | Cupriavidus necator | - |
succinate + NAD(P)H + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q0K2K1 | - |
- |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate precipitation and anion exchange chromatography and gel filtration | Cupriavidus necator |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Cupriavidus necator | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of 0.3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is sulfopropanal and NADP+ | Cupriavidus necator |
additional information | - |
specific activity of 0.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is succinale semialdehyde and NAD+ | Cupriavidus necator |
additional information | - |
specific activity of 0.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is succinale semialdehyde and NADP+ | Cupriavidus necator |
additional information | - |
specific activity of 0.6 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is sulfopropanal and NAD+ | Cupriavidus necator |
additional information | - |
specific activity of 0.7 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is succinale semialdehyde and NADP+ | Cupriavidus necator |
additional information | - |
specific activity of 10 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is sulfopropanal and NADP+ | Cupriavidus necator |
additional information | - |
specific activity of 2.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is sulfopropanal and NADP+ | Cupriavidus necator |
additional information | - |
specific activity of 3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is sulfopropanal and NAD+ | Cupriavidus necator |
additional information | - |
specific activity of 5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is sulfopropanal and NAD+ | Cupriavidus necator |
additional information | - |
specific activity of 5.0 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is succinale semialdehyde and NAD+ | Cupriavidus necator |
additional information | - |
specific activity of 7.0 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is succinale semialdehyde and NADP+ | Cupriavidus necator |
additional information | - |
specific activity of 7.3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is succinale semialdehyde and NAD+ | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-sulfopropanal + NAD(P)+ + H2O | - |
Cupriavidus necator | 3-sulfopropanoate + NAD(P)H + 2 H+ | - |
? | |
succinate semialdehyde + NAD(P)+ + H2O | - |
Cupriavidus necator | succinate + NAD(P)H + 2 H+ | - |
? | |
succinate semialdehyde + NAD+ + H2O | - |
Cupriavidus necator | succinate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
3-sulfopropanal dehydrogenase | - |
Cupriavidus necator |
GabD1 | - |
Cupriavidus necator |
NAD(P)+-coupled 3-sulfopropanal dehydrogenase | - |
Cupriavidus necator |
succinate-semialdehyde dehydrogenase | - |
Cupriavidus necator |
sulfopropanal dehydrogenase | - |
Cupriavidus necator |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | specific activity increases steadily to a broad optimum | Cupriavidus necator |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
enzyme is almost inactive | Cupriavidus necator |
Organism | Comment | Expression |
---|---|---|
Cupriavidus necator | transcript is detected inducibly in both homotaurine and GABA-grown cells | up |
General Information | Comment | Organism |
---|---|---|
metabolism | GABA metabolism | Cupriavidus necator |