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Literature summary for 1.2.1.13 extracted from

  • Wang, Y.; San, K.Y.; Bennett, G.N.
    Improvement of NADPH bioavailability in Escherichia coli by replacing NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase GapA with NADP+-dependent GapB from Bacillus subtilis and addition of NAD kinase (2013), J. Ind. Microbiol. Biotechnol., 40, 1449-1460 .
    View publication on PubMed
Search for more literature for 1.2.1.13

Cloned(Commentary)

Cloned (Comment) Organism
gene gapB, recombinant overexpression in and complementation of Escherichia coli gapA-deficient strain MG1655 gapA::Tn10, gene gapB is expressed from plasmid pDHC29 under the Plac promoter control, coexpression with Escherichia coli gene nadK encoding the NAD kinase, genes gapB and nadk are grouped as a single operon Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information to increase NADPH bioavailability, the native NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gapA gene, EC 1.2.1.12, in Escherichia coli is replaced with the NADP+-dependent gapB gene, EC 1.2.1.13, from Bacillus subtilis. To overcome the limitation of NADP+ availability, Escherichia coli NAD kinase, gene nadK is also coexpressed with gapB in Escherichia coli. replacing NAD+-dependent GapA activity with NADP+-dependent GapB activity increases the synthesis of NADPH-dependent compounds Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Bacillus subtilis
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Bacillus subtilis 168
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34425
-
-
Bacillus subtilis 168 O34425
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Bacillus subtilis 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Bacillus subtilis 168 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GapB
-
 Bacillus subtilis
NADP+-dependent gapB
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Bacillus subtilis