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Literature summary for 1.2.1.13 extracted from

  • Graciet, E.; Mulliert, G.; Lebreton, S.; Gontero, B.
    Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation (2004), Eur. J. Biochem., 271, 4737-4744.
    View publication on PubMed
Search for more literature for 1.2.1.13

Protein Variants

Protein Variants Comment Organism
K128A Km-value for NADPH is nearly identical to wild-type value, turnover-number for NADPH is decreased about 2fold. Km-value for NADH is increased 2.1fold compared to wild-type enzyme, turnover-number for NADH is decreased 2.6fold Chlamydomonas reinhardtii
K128E Km-value for NADPH is about 80% of the wild-type value, turnover-number for NADPH is decreased 2.7fold. Km-value for NADH is increased 2.3fold compared to wild-type enzyme, turnover-number for NADH is decreased 1.9fold Chlamydomonas reinhardtii
R197A Km-value for NADPH is identical to wild-type value, turnover-number for NADPH is about 90% of the wild-type value. Km-value for NADH is about 80% of the wild-type value, turnover-number for NADH is nearly identical to wild-type value Chlamydomonas reinhardtii
R197E Km-value for NADPH is increased 1.3fold compared to wild-type enzyme, turnover-number for NADPH is decreased 2fold. Km-value for NADH is about 90% of the wild-type value, turnover-number for NADH is increased 1.3fold Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 NADPH mutant enzyme K128E Chlamydomonas reinhardtii
0.027
-
 NADPH mutant enzyme K128A Chlamydomonas reinhardtii
0.028
-
 NADPH wild-type enzyme Chlamydomonas reinhardtii
0.028
-
 NADPH mutant enzyme R197A Chlamydomonas reinhardtii
0.035
-
 NADPH mutant enzyme R197E Chlamydomonas reinhardtii
0.099
-
 NADH mutant enzyme R197A Chlamydomonas reinhardtii
0.11
-
 NADH mutant enzyme R197E Chlamydomonas reinhardtii
0.12
-
 NADH wild-type enzyme Chlamydomonas reinhardtii
0.25
-
 NADH mutant enzyme K128A Chlamydomonas reinhardtii
0.272
-
 NADH mutant enzyme K128E Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADH
-
 Chlamydomonas reinhardtii D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?
3-phospho-D-glyceroyl phosphate + NADPH
-
 Chlamydomonas reinhardtii D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
GAPDH
-
 Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40
-
 NADH mutant enzyme K128A Chlamydomonas reinhardtii
56
-
 NADH mutant enzyme K128E Chlamydomonas reinhardtii
104
-
 NADH wild-type enzyme Chlamydomonas reinhardtii
108
-
 NADH mutant enzyme R197A Chlamydomonas reinhardtii
137
-
 NADH mutant enzyme R197E Chlamydomonas reinhardtii
161
-
 NADPH mutant enzyme K128E Chlamydomonas reinhardtii
220
-
 NADPH mutant enzyme K128A Chlamydomonas reinhardtii
220
-
 NADPH mutant enzyme R197E Chlamydomonas reinhardtii
392
-
 NADPH mutant enzyme R197A Chlamydomonas reinhardtii
430
-
 NADPH wild-type enzyme Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Chlamydomonas reinhardtii
NADPH
-
 Chlamydomonas reinhardtii