Cloned (Comment) | Organism |
---|---|
gene CgGAP, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, six stress-response elements are found in the upstream region of the CgGAP gene, phylogenetic analysis, funtional complementation of Saccharomyces cerevisiae transformed with CgGAP and restoration to the wild-type when cultured in high-osmolarity medium. Promoter studies in Saccharomyces cerevisiae using the GFP gene as a reporter show that the GAP promoter (PCgGAP) is constitutively expressed in Saccharomyces cerevisiae cells grown on D-glucose | Pichia kudriavzevii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Pichia kudriavzevii | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Pichia kudriavzevii WL2002-5 | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pichia kudriavzevii | - |
- |
- |
Pichia kudriavzevii WL2002-5 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Pichia kudriavzevii | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Pichia kudriavzevii WL2002-5 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the CgGAP protein consists of an N-terminal NAD+-binding domain and a central catalytic domain | Pichia kudriavzevii |
Synonyms | Comment | Organism |
---|---|---|
CgGAP | - |
Pichia kudriavzevii |
NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase | - |
Pichia kudriavzevii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pichia kudriavzevii |
General Information | Comment | Organism |
---|---|---|
metabolism | glyceraldehyde-3-phosphate dehydrogenase, GAPDH, is a key enzyme of glycolysis | Pichia kudriavzevii |