Literature summary for 1.2.1.12 extracted from

Sahoo, S.; Murugavel, S.; Devi, I.K.; Vedamurthy, G.V.; Gupta, S.C.; Singh, B.P.; Joshi, P.
Glyceraldehyde-3-phosphate dehydrogenase of the parasitic nematode Haemonchus contortus binds to complement C3 and inhibits its activity (2013), Parasite Immunol., 35, 457-467 .

Search for more literature for 1.2.1.12

Activating Compound

Activating Compound	Comment	Organism	Structure
ATP	required	Haemonchus contortus

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli	Haemonchus contortus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is part of the excretory-secretory products of the parasite	Haemonchus contortus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates	Haemonchus contortus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
140000	150000	native PAGE	Haemonchus contortus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	Haemonchus contortus	-	3-phospho-D-glyceroyl phosphate + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Haemonchus contortus	H6WVX3	propagated in sheep and goat, isolated from sheep and goat abomasums (stomachs)	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
adult	-	Haemonchus contortus	-
larva	-	Haemonchus contortus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.3	-	freshly isolated enzyme, complement-C3-binding protein activity, pH 8.0, temperature not specified in the publication	Haemonchus contortus
1	-	isolated enzyme from fresh parasites, GAPDH activity, pH 8.0, temperature not specified in the publication	Haemonchus contortus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Haemonchus contortus	3-phospho-D-glyceroyl phosphate + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
?	x * 14000, SDS-PAGE, probably truncated form, x * 37000, native enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE	Haemonchus contortus
More	isolation of a truncated form of the protein from the excretory-secretory products of the parasite worms using C3-affinity chromatography, and identification by mass spectroscopy as glyceraldehyde-3-phosphate dehydrogenase, GAPDH	Haemonchus contortus

Synonyms

Synonyms	Comment	Organism
complement-C3-binding protein	-	Haemonchus contortus
GAPDH	-	Haemonchus contortus
glyceraldehyde-3-phosphate dehydrogenase	-	Haemonchus contortus
H.c-C3BP	-	Haemonchus contortus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Haemonchus contortus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Haemonchus contortus
NADH	-	Haemonchus contortus

General Information

General Information	Comment	Organism
physiological function	the enzyme inhibits complement function as measured by haemolytic assay and membrane attack complex (MAC) formation, C3-binding property of Haemonchus contortus GAPDH is an additional function of the enzyme, and it represents another entity of complement-binding protein. Key role of the protein in immune modulation	Haemonchus contortus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)