Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | extracellular GAPDH in human serum is a multimeric, high-molecular-weight, yet glycolytically active enzyme. The enzyme is secreted | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Homo sapiens | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04406 | - |
- |
Purification (Comment) | Organism |
---|---|
extracellular serum isozyme by immunoaffinity purification combined with anion exchange chromatography by using DEAE affigel blue chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
colon carcinoma cell | - |
Homo sapiens | - |
HEP-3B cell | - |
Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
serum | a multimeric high-molecular-weight glyceraldehyde-3-phosphate dehydrogenase, the isozyme is secreted. Low levels of non-serum-specific isozyme GAPDH. The serum-specific high-molecular weight isozyme is also secreted from cancer cells | Homo sapiens | - |
SK-HEP-1 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | high-molecular-weight multimers of serum GAPDH, multiple protein bands (about 25, and 50-75 kDa) along with some proteins with weak signals in albumin-depleted serum samples, SDS-PAGE and mass spectrometry. The enzymatic function of serum GAPDH remained unaffected by the multimers | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Homo sapiens |
glyceraldehyde-3-phosphate dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | cellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phylogenetically conserved, ubiquitous enzyme | Homo sapiens |
physiological function | cellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phylogenetically conserved, ubiquitous enzyme that plays an indispensable role in energy metabolism. The extracellular GAPDH in human serum is a multimeric, high-molecular-weight, yet glycolytically active enzyme, the enzymatic function of serum GAPDH remained unaffected by the multimers | Homo sapiens |