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Literature summary for 1.2.1.12 extracted from

  • Fourrat, L.; Iddar, A.; Valverde, F.; Serrano, A.; Soukri, A.
    Effects of oxidative and nitrosative stress on Tetrahymena pyriformis glyceraldehyde-3-phosphate dehydrogenase (2007), J. Eukaryot. Microbiol., 54, 338-346.
    View publication on PubMed
Search for more literature for 1.2.1.12

Inhibitors

Inhibitors Comment Organism Structure
hydrogen peroxide maximum inhibition is observed at concentrations of 0.2 mM Tetrahymena pyriformis
additional information H2O2 does not significantly alter GAPDH-specific activity levels in cell free extracts Tetrahymena pyriformis
nitrogen oxide
-
 Tetrahymena pyriformis
NO diminishes GAPDH specific activity by 10%-20% Tetrahymena pyriformis
sodium nitroprusside maximum inhibition is observed at concentrations of 0.2 mM Tetrahymena pyriformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
 NAD+ in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 35°C Tetrahymena pyriformis
0.147
-
 D-glyceraldehyde 3-phosphate in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 35°C Tetrahymena pyriformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 subunit, SDS-PAGE Tetrahymena pyriformis

Organism

Organism UniProt Comment Textmining
Tetrahymena pyriformis
-
-
-
Tetrahymena pyriformis E / ATCC 30005
-
-
-

Oxidation Stability

Oxidation Stability Organism
the purified enzyme remains mostly unmodified or without any sign of degradation after exposure to NO or H2O2 Tetrahymena pyriformis

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information posttranslational modification of the enzyme by nitrogen oxide Tetrahymena pyriformis

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE-cellulose DE-52 column chromatography Tetrahymena pyriformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.72
-
 cell-free extract, in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 25°C Tetrahymena pyriformis
26.4
-
 after 36.7fold purifictaion, in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 25°C Tetrahymena pyriformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Tetrahymena pyriformis 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Tetrahymena pyriformis E / ATCC 30005 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
phosphorylating NAD+-dependent GAPDH
-
 Tetrahymena pyriformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Tetrahymena pyriformis

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent Tetrahymena pyriformis

pI Value

Organism Comment pI Value Maximum pI Value
Tetrahymena pyriformis isoelectric focusing
-
 8.8

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.05
-
 in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 35°C Tetrahymena pyriformis sodium nitroprusside
0.075
-
 in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 35°C Tetrahymena pyriformis hydrogen peroxide