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Literature summary for 1.2.1.12 extracted from
- Purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from European pilchard Sardina pilchardus (2007), Acta Biochim. Biophys. Sin. (Shanghai), 39, 947-954.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0734 | - |
D-Glyceraldehyde-3-phosphate | in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C | Sardina pilchardus |  |
| 0.092 | - |
NAD+ | in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25°C | Sardina pilchardus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sardina pilchardus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
4 * 37000, denaturing SDS-PAGE | Sardina pilchardus |
| 38500 | - |
4 * 38500, non-denaturing PAGE | Sardina pilchardus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sardina pilchardus | - |
European pilchard | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation and Blue Sepharose column chromatography | Sardina pilchardus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Sardina pilchardus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.46 | - |
crude extract | Sardina pilchardus |
| 35.75 | - |
after 77.71fold purification | Sardina pilchardus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Sardina pilchardus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 37000, denaturing SDS-PAGE | Sardina pilchardus |
| homotetramer | 4 * 38500, non-denaturing PAGE | Sardina pilchardus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAPDH | - |
Sardina pilchardus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Sardina pilchardus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | 32 | - |
Sardina pilchardus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 60 | pre-incubation for 10 min at temperatures varying between 15°C and 30°C does not irreversibly affect the enzyme activity, thermal inactivation occurs above 35°C and results in total activity loss at 60°C | Sardina pilchardus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Sardina pilchardus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Sardina pilchardus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | dependent | Sardina pilchardus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sardina pilchardus | isoelectric focusing | - |
7.9 |
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