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Literature summary for 1.2.1.12 extracted from

  • Terao, Y.; Yamaguchi, M.; Hamada, S.; Kawabata, S.
    Multifunctional glyceraldehyde-3-phosphate dehydrogenase of Streptococcus pyogenes is essential for evasion from neutrophils (2006), J. Biol. Chem., 281, 14215-14223.
    View publication on PubMed
Search for more literature for 1.2.1.12

Application

Application Comment Organism
medicine the Plr-SDH-GAPDH complex participates in invasive streptococcal infections Streptococcus pyogenes

Cloned(Commentary)

Cloned (Comment) Organism
a 1008 bp DNA fragment from the plr-sdh-gapdh gene is ligated into the vector pGEX-6P-1 for expression of the protein in Escherichia coli BL212DE3 cells Streptococcus pyogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface in complex with the streptococcal plasmin receptor, Plr, and streptococcal surface dehydrogenase, SDH Streptococcus pyogenes 9986
-
extracellular the Plr-SDH-GAPDH complex is expressed on the bacterial surface and released from cells Streptococcus pyogenes
-
-

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes P0C0G6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein is purified using glutathione-Sepharose 4B and PreScission protease Streptococcus pyogenes

Synonyms

Synonyms Comment Organism
GAPDH
-
 Streptococcus pyogenes
glyceraldehyde-3-phosphate dehydrogenase
-
 Streptococcus pyogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 the enzymatic activity of cell-associated Plr-SDH-GAPDH complex is measured Streptococcus pyogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 the enzymatic activity of cell-associated Plr-SDH-GAPDH complex is measured Streptococcus pyogenes